| 低强度超声对鼻咽癌CNE2细胞增值和凋亡的影响 |
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| 引用本文： | 王萍,夏新蜀,王志刚,余和平,白定群,徐静,王昕娜,何勇,谭勇,向君彦,许川山. 低强度超声对鼻咽癌CNE2细胞增值和凋亡的影响[J]. 激光杂志, 2009, 30(2): 76-77 |
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| 作者姓名： | 王萍 夏新蜀 王志刚 余和平 白定群 徐静 王昕娜 何勇 谭勇 向君彦 许川山 |
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| 作者单位： | 重庆医科大学附属第二医院超声影像研究所,重庆,400010;重庆医科大学附属第一医院康复医学科,重庆,400016 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 目的：观察低强度超声对人鼻咽癌CNF2细胞增殖和凋亡的影响。方法：以低强度超声（频率1．7MHz，剂量1．35W／cm2）辐照CNF2细胞，采用MTT观察超声对COCl细胞增殖的抑制作用；光镜观察细胞形态，细胞核荧光染色法检测凋亡细胞。结果：超声波能够显著抑制鼻咽癌CNE2细胞增殖，辐照后孵育18h，其MTT光吸收值与对照组相比监著降低：光镜下可见细胞形态学改变，同时Hoechst 33258荧光染色法观察到亮蓝色着染的凋亡细胞。结论：低强度超声辐照对人鼻咽癌CNE2细胞体外生长具有明显的抑制作用，并可诱导其凋亡。
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| 关 键 词： | 低强度超声 细胞凋亡 CNE2细胞 |
Influence of low intensity ultrasound on proliferation and apoptosis of human nasopharyngeal CNE2 |
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| WANG Ping,XIA Xin-shu,WNAG Zhi-gang,YU He-ping,BAI Ding-qun,XU Jing,WANG Xin-na,HE Yong,TAN Yong,XIANG Jun-yan,XU Chuan-shan. Influence of low intensity ultrasound on proliferation and apoptosis of human nasopharyngeal CNE2[J]. Laser Journal, 2009, 30(2): 76-77 |
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| Authors: | WANG Ping XIA Xin-shu WNAG Zhi-gang YU He-ping BAI Ding-qun XU Jing WANG Xin-na HE Yong TAN Yong XIANG Jun-yan XU Chuan-shan |
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| Affiliation: | WANG Ping1,XIA Xin-shu2,WNAG Zhi-gang1,YU He-ping2,BAI Ding-qun2,XU Jing1,WANG Xin-na1,HE Yong1,TAN Yong2,XIANG Jun-yan1,XU Chuan-shan1 1.Institute of Ultrasonic Engineering in Medicine,Chongqing University of Medical Sciences,Chongqing 400010,China,2.Department of Rehibiliation Medicine,First Affiliated Hospital,Chongqing 400016 |
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| Abstract: | Objective:To investigate the effect of low-intensity ultrasound on proliferation and apoptosis of human cell line CNE2.Methods:The growth inhibitory effect of Ultrasound Exposure(1.7MHz,1.35W/cm2)on CNE2 cell was observed by MTF colormetric assay.Light microscope was applied to detect the morphological changes of CNE2 cell.Cell nucleus fluorescent staining was applied to detect the cell apoptosis.Results:Sonication could obviously inhibit the proliferation of CNE2 cell.The absorbance value(A570)of MTT signi... |
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| Keywords: | low intensity ultrasound apoptosis CNE2 cell |
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