血红蛋白作催化剂茜素蓝S褪色法测定过氧化氢

H2O2能够氧化茜素蓝S使之褪色,而模拟酶——血红蛋白对其具有催化作用。据此建立了一种以茜素蓝S为指示剂的过氧化氢——茜素蓝S——血红蛋白酶催化反应体系测定痕量H2O2的新方法。研究了反应的最佳条件,体系的酸度为pH=10.1的NH3-NH4Cl缓冲溶液,最大吸收波长为580nm。该法的线性范围为2.0×10-7~7.5×10-5mol/L,检出限为1.3×10-8mol/L,表观摩尔吸光系数为1.7×104L/(mol·cm)。该方法可用于雨水及消毒水中H2O2的测定。结果今人满意。该方法简单、灵敏、选择性好,易于操作。

Fading Spectrometric Method for the Determination of Hydrogen Peroxide with Alzarin Blue S Using Hemoglobin as Catalyst

A new enzymatic catalytic method was developed for the determination of trace hydrogen peroxide using hemoglobin as catalyst.The color of alizarin blue S was faded by oxidation with hydrogen peroxide.The oxidation was enhanced in the presence of hemoglobin.The optimum conditions and effect of foreign ions were discussed.At pH 10.1 of NH_3-NH_4Cl buffer solution,the system exhibited maximum absorption wavelength at 580 nm.Under the optimal conditions,the degree of fading(ΔA) was linear to the H_2O_2 concentration(c) in the range of 2.0×10-7～7.5×10-5 mol/L,and the apparent molar absorptivity ε_525 was 1.7×104 L/(mol·cm) with the detection limit of 1.3×10-8 mol/L.This method is simple,sensitive and suitable for the determination of hydrogen peroxide in rain and disinfector with satisfactory results.