麸皮对里氏木霉Rut C-30产纤维素酶的促进作用

本研究尝试通过里氏木霉RutC-30添加适量的麸皮以及利用实验设计软件Design-Expert寻找适宜的麸皮与微晶纤维素的配比来研究麸皮对里氏木霉RutC-30产纤维素酶的影响。结果表明，适当的麸皮添加量能够促进纤维素酶的生产：通过二元二次正交旋转组合设计，确定了微晶纤维素添加量和麸皮添加量分别为12．23和23．50g/L的优化产酶条件：此奈件下，在250mL摇瓶中滤纸酶活达到6．383FPIU／mL，得率系数为521．913FPIU／g；在7．5L发酵罐中，滤纸酶活为6．807FPIU／mL，得率系数为556．582FPIU／g。相比于优化前，优化后摇瓶实验和发酵罐实验中滤纸酶活分别提高了14．247％和17．403％，而纤维素酶的得率系数却分别降低了6．584％和4．005％。分别以酸解杨木残渣和蒸汽爆破杨木浆替代微晶纤维素作为碳源，最终获得最高滤纸酶活1．953FPIU／mL和1．745FPIU／mL。

Enhancement of Bran to CeUulase Production by Trichoderma reesei Rut C-30

The effect of bran content on cellulase production by Trichoderma reesei Rut C-30 was studied through adding bran in appropriate amounts and investigating suitable ratio of bran to microcrystalline cellulose by software of Design-Expert. The results showed that bran in appropriate amounts could accelerate the production of cellulase. The optimal conditions for cellulase production obtained by the design of rotation-regression-orthogonal combination were 12.23 g/L of microcrystalline cellulose and 23.50 g/L of bran. Filter paper activity and yield coefficient in 250 mL shake flask was 6. 383 FPIU/mL and 521. 913 FPIU/g, respectively. Filter paper activity and yield coefficient in 7.5 L fermentor was 6. 807 FPIU/mL and 556.582 FPIU/g, respec- tively. Filter paper activity in shake flask and fermentor increased 14.247 % and 17. 403 %, after optimization, respectively. Yield of cellulase decreased 6.584 % and 4.005 %, respectively. Filter paper activity were 1. 953 FPIU/mL and 1. 745 FPIU/mL, when replacing microcrystalline cellulose with dilute acid pretreated poplar and steam exploded poplar, respectively.