乙酰胆碱对小剂量辐射小鼠脾细胞增殖反应的影响

采用７５ｍＧｙ Ｘ射线全身和离体照射昆明系雄性小鼠脾细胞，观察乙酰胆碱对其增殖反应的影响。其结果发现，Ａｃｈ增强脾细胞自发增殖能力及对ＣｏｎＡ５反应性；７５ｍＧｙ Ｙ射线全身和离体照射后脾细胞增殖反应变化不同，全身照射后早期增殖更为显著，而离体照射时似较未受照射细胞为差。

EFFECTS OF Ach ON PROLIFERATIVE REACTION OF MURINE SPLENOCYTES RADIATED WITH LOW DOSE RADIATION

The present study was designed to observe the effects of acetylcholine (Ach) on the proliferative reaction of the splenocytes irradiated with low dose X-rays in Kunmin male mice in vivo and in vitro. The results showed that Ach enhanced significantly the spontaneous proliferations of the splenocytes and the responses of the splenocytes to ConA. But. the immune functional changes of the splenocytes irradiated with 75 mGy in vivo and in vitro were different. It would increase more in vivo. while less in vitro. These results suggest that Ach may stimulate the splenocyte proliferative reaction and participate in the immunoenhanced effect following low dose radiation to a certain extent. However, the action mechanism of Ach on the immune functional changes of the splenocytes irradiated with low dose X-rays in vivo and in vitro may be different. It is supposed to have relation to the change of the microenvironment of the splenocytes.