| 反相高效液相色谱法测定苹果醋中的有机酸 |
| |
| 引用本文： | 赵芳,孙国伟,郭志伟. 反相高效液相色谱法测定苹果醋中的有机酸[J]. 广州化工, 2012, 40(18): 92-93,145 |
| |
| 作者姓名： | 赵芳 孙国伟 郭志伟 |
| |
| 作者单位： | 1. 焦作出入境检验检疫局实验中心,河南焦作,454002
2. 焦作市质量技术监督检验测试中心,河南焦作,454002 |
| |
| 摘 要： | 用反相高效液相色谱法测定了苹果醋饮料、苹果原醋等样品中的主要有机酸含量。通过C18柱进行分离和测定,以0.01 mol/L磷酸氢二铵-磷酸缓冲溶液作流动相,流速1.0 mL/min,紫外检测器210 nm处进行检测。实验表明,苹果原汁中的有机酸含量最高,样品回收率为90.8%～99.5%之间。证明该方法的准确性较高,完全适用于苹果醋中有机酸的检测分析。
|
| 关 键 词： | 高效液相色谱法 有机酸 苹果醋 |
Determination of Organic Acid in Apple Vinegar by Reversed Phase High Performance Liquid Chromatography |
| |
| ZHAO Fang,SUN Guo-wei,GUO Zhi-wei. Determination of Organic Acid in Apple Vinegar by Reversed Phase High Performance Liquid Chromatography[J]. GuangZhou Chemical Industry and Technology, 2012, 40(18): 92-93,145 |
| |
| Authors: | ZHAO Fang SUN Guo-wei GUO Zhi-wei |
| |
| Affiliation: | ZHAO Fang , SUN Guo - wei, GUO Zhi - wei ( 1 Jiaozuo Enter - Exit Inspection and Quarantine Bureau, Henan Jiaozuo 454001 ; 2 Jiaozuo Quality and Technical Supervision Bureau, Henan Jiaozuo 454001, China) |
| |
| Abstract: | Determination of organic acid in apple vinegar beverage and apple vinegar by reversed phase high per- formance liquid chromatography was studied. The separation was performed on a Cls column with 0. 01 mol/L diammonium phosphate - phosphate buffer solution as mobile phase, the/low rate and detection wavelengths were set to be 1.0 mL/min and 210 nm, respectively. Experimental results showed that the maximum content of organic acid was obtained in the ap- ple juice, and the sample recovery rate was in the range of 90. 8% -99.5%. It was proved that the accuracy of this meth- od was very high and it＇s completely applicable to test the contents of organic acid in apple vinegar. |
| |
| Keywords: | high performance liquid chromatography organic acid apple vinegar |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
