| 杂色云芝漆酶用于提取人参总皂甙的研究 |
| |
| 引用本文： | 郭梅,白东清,蒲军,杜连祥,路福平.杂色云芝漆酶用于提取人参总皂甙的研究[J].食品科学,2006,27(2):166-168. |
| |
| 作者姓名： | 郭梅 白东清 蒲军 杜连祥 路福平 |
| |
| 作者单位： | 天津农学院食品科学系； 天津科技大学生物工程学院工业微生物重点实验室 |
| |
| 摘 要： | 采用正交试验,对水浸提取条件、漆酶酶解条件进行了研究。结果表明人参总皂甙最佳提取工艺为:先用漆酶酶解,每克人参加入15ml漆酶粗酶液(92U/ml),在30℃、pH3.5的条件下酶解1.5h,然后水浸提取人参总皂甙。经过漆酶酶解处理后人参总皂甙提取率比水浸提取法提高了70.9%。
|
| 关 键 词： | 漆酶 酶解 人参总皂甙 正交试验 |
| 文章编号： | 1002-6630（2006）02-0166-03 |
| 收稿时间： | 2005-02-26 |
| 修稿时间： | 2005-02-26 |
Extraction Study on Ginsenosides with Laccase |
| |
| GUO Mei,BAI Dong-qing,PU Jun,DU Lian-xiang,LU Fu-ping.Extraction Study on Ginsenosides with Laccase[J].Food Science,2006,27(2):166-168. |
| |
| Authors: | GUO Mei BAI Dong-qing PU Jun DU Lian-xiang LU Fu-ping |
| |
| Affiliation: | 1.Department of Food Science, Tianjin Agricultural College, Tianjin 300384, China; 2.Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300222, China;3. Key Laboratory of Ecology and Aquaculture, Department of Fishery Science, Tianjin Agricultural College, Tianjin 300384, China |
| |
| Abstract: | The optimum conditions of enzymolysis and extraction process by orthogonal design were studied. The results showed that the optimum conditions of ginsenosides extraction technique were as follows: First, laccase treatment: 1g Panax ginseng was put into 15ml (92U/ml) laccase with temperature 30℃, pH value 3.5 and fermenting time 1.5h. Then, in comparison with water-extraction, the enzyme-based extraction rate of ginsenosides is increased by 70.9%. |
| |
| Keywords: | laccase enzymolysis ginsenosides orthogonal design |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《食品科学》浏览原始摘要信息 |
|
点击此处可从《食品科学》下载全文 |
|
