| 苹果汁中耐热菌培养基的优化及生长曲线 |
| |
| 引用本文： | 关健,岳田利,薛淑静,袁亚宏,蔡福带.苹果汁中耐热菌培养基的优化及生长曲线[J].食品工业科技,2006(5):100-102. |
| |
| 作者姓名： | 关健 岳田利 薛淑静 袁亚宏 蔡福带 |
| |
| 作者单位： | 西北农林科技大学食品科学与工程学院,陕西杨凌,712100 |
| |
| 基金项目： | 西部开发科技行动项目;科技部科研项目;教育部霍英东教育基金 |
| |
| 摘 要： | 通过对苹果汁中分离耐热菌和德国标准耐热菌菌株基础培养基的正交优化,确定最佳的培养基。得出最佳培养基配比是(质量分数):德国标准菌:蛋白胨0.5%,果糖0.2%,酵母粉0.2%,MgSO4·2H2O0.1%,CaCl20.5%,KH2PO40.12%,MnSO4·4H2O0.05%;分离菌:蛋白胨0.5%,葡萄糖0.2%,酵母粉0.2%,MgSO4·2H2O0.1%,CaCl20.5%,KH2PO40.12%,MnSO4·4H2O0.05%。并计算得出吸光度与细菌浓度之间的关系公式及用优化培养基培养绘制耐热菌生长曲线。
|
| 关 键 词： | 苹果汁 耐热菌 培养基优化 吸光度 生长曲线 |
| 文章编号： | 1002-0306(2006)05-0100-03 |
| 修稿时间： | 2006年1月11日 |
Optimization of culture medium and growth curve of thermoacidophilic bacteria in apple juice |
| |
| Guan Jian.Optimization of culture medium and growth curve of thermoacidophilic bacteria in apple juice[J].Science and Technology of Food Industry,2006(5):100-102. |
| |
| Authors: | Guan Jian |
| |
| Affiliation: | Guan Jian et al |
| |
| Abstract: | The optimization experiment of culture medium of the isolated thermo-acidophilic bacteria and the standard thermo-acidophilic(purchase from German)was conducted by quadratic design. The best medium composition was as following:peptone 0.5%,fructose 0.2%,yeast power 0.2%,MgSO4.2H2O 0.1%,CaCl2 0.5%,KH2PO4 0.12%, MnSO4.4H2O 0.05% for standard Thermo-acidophilic and peptone 0.5%,glucose 0.2%,yeast power 0.2%,MgSO4.2H2O 0.1%,CaCl2 0.5%,KH2PO4 0.12%, MnSO4.4H2O 0.05% for the isolated Thermo-acidophilic.The formula of absorbency and bacterial concentration was got. And thermo-acidophilic growth curve with optimized culture was determined. |
| |
| Keywords: | apple juice Thermo-acidophilic bacteria culture medium optimization absorbency growth curve |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
