| 免疫金标记肿瘤细胞表面抗原的软X射线接触显微术观察 |
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| 引用本文： | 魏道严,徐文轩.免疫金标记肿瘤细胞表面抗原的软X射线接触显微术观察[J].核技术,1996,19(1):41-43. |
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| 作者姓名： | 魏道严 徐文轩 |
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| 作者单位： | 安徽医科大学，中国科学院高能物理研究所同步辐射国家重点实验室 |
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| 摘 要： | 在建立了适于软Ｘ射线接触显微术观察的细胞样品免疫细胞化学染色技术的基础上，对培养的Ｈｅｐ－２肿瘤细胞表面ＨＬＡ－Ａ２抗原进行免疫金银染色，并应用ＳＸＣＭ观察。结果显示；肿瘤细胞表面抗原的免疫金银染色颗粒图像反差明显。立体感强，大小和形态各异的颗粒清晰易辨。
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| 关 键 词： | 肿瘤细胞 免疫金银染色 表面抗原 SXCM |
Observation of immunogold labelled tumor cell surface antigens by soft X-ray contact microscopy |
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| Wei Daoyan, Shen Hengjia.Observation of immunogold labelled tumor cell surface antigens by soft X-ray contact microscopy[J].Nuclear Techniques,1996,19(1):41-43. |
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| Authors: | Wei Daoyan Shen Hengjia |
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| Abstract: | After establishing the immuno-cytochemical staining technique of cell samples suitable for soft X-ray contact microscopy(SXCM), the HLA-A2 surface antigens of cultured Hep-2 tumor cells were labelled by immunogold silver staining(IGSS) and detected by SXCM. The resultS reveal that the immunogold silver granules on the surface of Hep-2 tumor cells have sharp contrast and show stereo features, and the granules with different sizes and shapes can be clearly seen and easily identified. |
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| Keywords: | Soft X-ray contact microscopy Tumor cell Immunogold silver staining Surface antigen |
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