| 黄芪多糖的分离纯化和抗肿瘤活性研究 |
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| 引用本文： | 朱振元,刘荣强,周芳,李盛峰,原静. 黄芪多糖的分离纯化和抗肿瘤活性研究[J]. 现代食品科技, 2011, 27(4): 376-379 |
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| 作者姓名： | 朱振元 刘荣强 周芳 李盛峰 原静 |
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| 作者单位： | 食品营养与安全教育部重点实验室,天津科技大学食品工程与生物技术学院,天津,300457 |
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| 基金项目： | 天津市科技计划项目应用基础与前沿技术研究计划重点项目，天津市高等学校科技发展基金计划项目 |
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| 摘 要： | 在不同因素水平下优化黄芪多糖水煮醇沉法的提取条件,得出黄芪多糖最佳提取条件为提取时间2 h,提取温度80℃,料液比1:10,多糖得率为9.77%;利用醇沉、DEAE-Sephadex A-25和Sephadex G-100进行分离纯化,得到两种黄芪多糖,纯度和糖含量较高,效果较好;抗肿瘤活性表明,黄芪多糖对H22腹水瘤...
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| 关 键 词： | 黄芪多糖 提取 纯化 抗肿瘤 |
Extraction and Purification of Astragalus Polysaccharide and its Anti-tumor Activity |
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| ZHU Zhen-yuan,LIU Rong-qiang,ZHOU Fang,LI Sheng-feng,YUAN Jing. Extraction and Purification of Astragalus Polysaccharide and its Anti-tumor Activity[J]. Modern Food Science & Technology, 2011, 27(4): 376-379 |
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| Authors: | ZHU Zhen-yuan LIU Rong-qiang ZHOU Fang LI Sheng-feng YUAN Jing |
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| Affiliation: | (Key Laboratory of Food Nutrition and Safety(Tianjin University of Science & Technology,Ministry of Education,College of Food Science and Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China) |
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| Abstract: | Different factors and levels were used for the Astragalus polysaccharide extraction by water bath.The results showed that the best ratio of solid to liquid,extraction temperate and time were in 1:10,80 ℃ and 2 h,respectively,under which the extraction rate could reach 9.77% under such conditions.Two types of Astragalus polysaccharide were obtained after alcohol precipitate and purification of DEAE-Sephadex A-25 and Sephadex G-100.The result of anti-tumor activity research showed that at dose of 50 mg/kg,100 mg/kg,the inhibitory rates of two kinds of polysaccharide were 49.81%,55.47%,45.46% and 47.72%. |
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| Keywords: | Astragalus polysaccharide extraction purification anti-tumor |
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