碱提酸沉法提取茶叶蛋白质的研究

以绿茶为原料,采用碱提酸沉的方法提取茶叶蛋白质,以料液比、碱液浓度、温度、时间为考查因素,研究茶叶蛋白质的最佳提取工艺条件,并对提得的茶叶蛋白质进行了氨基酸分析.结果表明:碱提酸沉法提取茶叶蛋白质的最佳工艺条件为料液比1∶25(m/V),碱液浓度03 mol/L,提取温度50℃,提取时间1 h,连续提取两次,茶叶蛋白质...

Extraction of Tea Protein using Alkali Extraction-acid Precipitation Method

Extraction of tea-protein from green tea by using alkali extraction and acid precipitation was investigated.Amino acid compositions of the tea protein ware also analyzed.The results showed that the optimum extraction parameters were NaOH 0.3 mol/L,green tea/water solution(m/V) 1:25,temperature50 ℃,stirring time 1 h and extraction times twice,under which the protein extraction efficiency was 85.50%.The best pI of the tea protein was 3.0,the protein concentration precipitation rate was 67.85% and the protein content was 47.76%.The tea protein contained 18 kinds of amino acid,in which essential amino acids were 40.68%.Amino acid score of tea protein was higher than soybean protein and close to those of breast milk and milk,which indicated that tea protein was one of high quality leaf proteins.This method is easy to operate,low-cost and highly efficient,thereby being suitable for the massive industrial production of tea protein.