| 蚕蛹蛋白酶解制备抗氧化肽的初步研究 |
| |
| 引用本文： | 李高扬,崔堂兵,陈亮. 蚕蛹蛋白酶解制备抗氧化肽的初步研究[J]. 现代食品科技, 2011, 27(7): 810-814 |
| |
| 作者姓名： | 李高扬 崔堂兵 陈亮 |
| |
| 作者单位： | 华南理工大学生物科学与工程学院,广东广州,510006 |
| |
| 摘 要： | 将蚕蛹通过粉碎和丙酮脱脂后,得到蚕蛹蛋白粉.用碱性蛋白酶、中性蛋白酶、胃蛋白酶、木瓜蛋白酶、胰蛋白酶酶解蚕蛹蛋白粉,以DPPH清除能力为指标对酶解过程进行分析,结果表明胃蛋白酶对蚕蛹蛋白有较好的酶解效果.然后通过正交优化胃蛋白酶的酶解条件,并研究水解产物多肽的体外抗氧化活性.
|
| 关 键 词： | 蚕蛹蛋白 酶解 抗氧化 肽 |
Preliminary Study of Enzymatic Hydrolysis of Silkworm Pupa Protein for Production of Antioxidant peptide |
| |
| LI Gao-yang,CUI Tang-bing,CHEN Liang. Preliminary Study of Enzymatic Hydrolysis of Silkworm Pupa Protein for Production of Antioxidant peptide[J]. Modern Food Science & Technology, 2011, 27(7): 810-814 |
| |
| Authors: | LI Gao-yang CUI Tang-bing CHEN Liang |
| |
| Affiliation: | (School of Bioscience and Bioengineering,South China University of Technology,Guangzhou 51006,China) |
| |
| Abstract: | Silkworm pupa was crushed and defatted by acetone,giving silkworm pupa powder.Then the powder was hydrolyzed with five proteases(Alkali protease,neutral protease,pepsin,papain,trypsin).Among the tested enzymes,pepsin was confirmed as an optimal enzyme preparation to prepare silkworm pupa polypeptide in terms of DPPH.scavenging activity of hydrolysates.The enzymatic hydrolysis procedure was optimized using orthogonal array method and the corresponding product was subjected to further analysis of antioxidant activity,such as reducing power and O2.,.OH and DPPH.scavenging activities. |
| |
| Keywords: | silkworm pupa protein enzyme hydrolysis antioxidation peptide |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
