夏枯草水溶性酸性多糖的分离及活性分析

采用水提醇沉法提取夏枯草多糖，然后经非极性吸附树脂HZ-820 脱色和脱蛋白后，再用DEAE-SepharoseFast Flow 阴离子交换柱层析进行分离得到酸性多糖。化学法和红外光谱证明该酸性多糖中半乳糖醛酸和硫酸根含量较高，间羟基联苯- 硫酸和氯化钡- 明胶比浊法测定其半乳糖醛酸和硫酸根含量分别为(58.85 ± 0.46)% 和(7 ± 0.52)%。紫外、红外光谱分析结果发现，该酸性杂多糖为非糖蛋白，可能含半乳糖醛酸、木糖、葡萄糖、鼠李糖和半乳糖等单糖。抗氧化活性研究表明该酸性多糖具有显著的DPPH 自由基和羟自由基清除活性。

Isolation and Antioxidant Activity of Water-soluble Acidic Polysaccharide from Prunella vulgaris Linn

An acidic polysaccharide with antioxidant activity was obtained from Prunella vulgaris Linn through sequential steps of water extraction and alcohol precipitation, then decolorization and deproteinization by HZ-820-type unpolar resin adsorption, and finally purification. 3-Hydroxydiphenyl-sulfuric acid and barium chloride-gelatin turbidimetric methods were used to determine contents of galacturonic acid and sulfate in the polysaccharide, and the results were (58.85 ± 0.46)% and (7 ± 0.52)%, respectively. Ultraviolet and infrared (IR) spectral analyses indicated that the monosaccharide composition of the polysaccharide consisted of galacturonic acid, xylose, glucose, rhamnose, and galactose and it belonged to the nonglycoprotein family. Moreover, this polysaccharide revealed strong scavenging activities against hydroxyl free radicals and DPPH free radicals.