| L-谷氨酰肼为底物酶法制备茶氨酸 |
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| 引用本文： | 张飞,熊吉滨,刘均忠,赵根海,焦庆才.L-谷氨酰肼为底物酶法制备茶氨酸[J].精细化工,2010,27(2). |
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| 作者姓名： | 张飞 熊吉滨 刘均忠 赵根海 焦庆才 |
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| 作者单位： | 1. 南京大学,生命科学学院,医药生物技术国家重点实验室,江苏,南京,210093;包头轻工职业技术学院,生物工程系,内蒙古,包头,014043
2. 南京大学,生命科学学院,医药生物技术国家重点实验室,江苏,南京,210093 |
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| 基金项目： | 国家技术创新基金资助项目(02CJ-13-01-16)~~ |
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| 摘 要： | 采用一种廉价的底物L-谷氨酰肼代替L-谷氨酰胺,成功地合成了茶氨酸,并且对该酶促反应的条件进行了初步优化。结果表明,以L-谷氨酰肼为底物时,γ-谷氨酰转肽酶(GGT)活性约是以L-谷氨酰胺为底物时的78.3%。该酶促反应的最适条件为:pH=10,反应温度40℃,n(L-谷氨酰肼)∶n(乙胺)=1∶10,L-谷氨酰肼初始浓度0.3mol/L。利用以上条件进行了茶氨酸的小量制备,投料L-谷氨酰肼50g,乙胺140g,反应40h,L-谷氨酰肼的摩尔转化率达84.1%,经离子交换树脂分离产物,得到31.8g高纯度茶氨酸。该结果显示良好应用前景。
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| 关 键 词： | L-谷氨酰肼 茶氨酸 γ-谷氨酰转肽酶 酶法合成 生物工程 |
Enzymatic Synthesis of Theanine with L-Glutamlhydrine as Substrate |
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| ZHANG Fei,XIONG Ji-bin,LIU Jun-zhong,ZHAO Gen-hai,JIAO Qing-cai.Enzymatic Synthesis of Theanine with L-Glutamlhydrine as Substrate[J].Fine Chemicals,2010,27(2). |
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| Authors: | ZHANG Fei XIONG Ji-bin LIU Jun-zhong ZHAO Gen-hai JIAO Qing-cai |
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| Affiliation: | 1.State Key Laboratory of Pharmaceutical Biotechnology/a>;Nanjing University/a>;Nanjing 210093/a>;Jiangsu/a>;China/a>;2.Baotou Light Industry Vocational Technical College/a>;Baotou 014043/a>;Inner Mongolia/a>;China |
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| Abstract: | In this research,an economical substrate,L-glutamlhydrine,was used in theanine synthesis catalyzed by Escherichia coli cells with L-glutamyltranspeptidase(GGT) activity.The result showed that GGT activity with L-glutamlhydrine as substrate was about 78.3% of that with L-glutamine as substrate.In the optimized reaction condition with 0.3 mol/L L-glutamlhydrine,3 mol/L ethylamine and 0.018 g/mL of GGT cells at pH=10 and 40 ℃,0.25 mol/L theanine was obtained in 40 h.The conversion rate from L-glutamlhydrine to theanine was 84.1%.Theanine of high purity was obtained by ion exchange resin.The results exhibited a good prospect of application. |
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| Keywords: | L-glutamlhydrine theanine enzymatic synthesis biological engineering |
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