微型化DNS法测定多糖水解液中还原糖的质量浓度

传统DNS比色法适用于常规还原糖含量的检测,但难以检测微量的样品以及快速检测大批量样品,故对传统DNS法进行改进,建立微型化DNS法测定多糖水解液中还原糖含量的方法.以DNS为显色剂,采用酶联免疫测定仪微盘比色测定多糖水解液中的还原糖含量,考察该方法的重复性和精确性,并与传统DNS法进行比较,结果表明:微型DNS法标准曲线线性良好,并且有良好的重复性和精密度,与传统DNS法相比,对样品的测定结果进行t检验,P=0.118 1(＞0.05)表明两种方法检测到结果相一致.微型化DNS法在保证检测精确性的前提下有效减少了样品检测损耗量和提高了检测效率,有望扩大DNS比色法至微量化及批量化样品的还原糖含量检测领域.

Miniaturization for determination of reducing sugar mass concentration using 3,5-dinitrosalicylic acid method

Traditional DNS method for determination of reducing sugar can not deal with rapid detection and microanalysis.There is need to improve traditional DNS method.To miniaturize the DNS method for determination of reducing sugar content in hydrolysates of polysaccharide.Reducing sugar in hydrolysates of polysaccharide,which were coloured by 3,5-dinitrosalicylic acid(DNS),were determined by microplate spectrophotometer.The method demonstrated considerable linearity,repeatability and accuracy.Compared with traditional DNS method in determining of reducing sugar by t test,P=0.118 1(>0.05),this indicated that two methods have the same result in determining of reducing sugar.The method by microplate spectrophotometer was markedly convenient,rapid,and small sample consumption,especially suitable for the determination of large number of microliter samples.