天津地区梭子蟹中过敏原组分分析

目的 分析天津地区梭子蟹蛋白提取液中主要过敏原组分特征.方法 常规方法制备蟹蛋白粗提液,经SDS-PAGE技术分析蛋白组分；分别以蟹过敏患者混合血清(sIgE)和单例血清(sIgE)为探针,应用Western-blot技术鉴别提取液中主要过敏原组分,同时观察不同患者过敏原组分的异质性.结果 蟹提取蛋白液SDS-PAGE结果显示,共有9条蛋白带,其中以70和74kD组分含量较高；Western-blot结果显示,除21、38kD外,其余多种蛋白组分均能与混合阳性血清反应,同时证实不同蟹过敏患者之间过敏原组分具有明显异质性.结论 天津地区蟹主要的过敏原是36、48、94kD三种蛋白,但不同的个体间主要过敏蛋白存在差异性.

Analysis of allergen components from swimming crab Portunus trituberculatus in Tianjin region

Objective To analyze the characteristics of major allergen components from protein extracts of swimming crab Portunus trituberculatus in Tianjin region.Methods Crude protein extracts were prepared from the crab and separated by SDS-PAGE,then transferred to PVDF membrane and incubated with patients’ specific IgE(sIgE) serum mixture and individual patient’s sIgE serum,respectively.After incubated with the second antibody,the reactivity was detected among different patients.Results SDS-PAGE showed 9 protein bands in the extracts,of which 70 and 74kD were at high level.Western blot showed that patients’ sIgE serum mixture could react with the majority of proteins in the extracts except 21 and 38kD proteins.The allergens in different patients were heterogeneous.Conclusion Three major allergens(36,48 and 94kD protein) were identified in the extracts of swimming crab Portunus trituberculatus from Tianjin,but there was also individual difference.