|
|||||
|
|
Methodological approaches for histamine quantification using derivatization by chloroethylnitrosourea and ELISA measurement: Part I. Optimization of derivated histamine detection with coated-plates using optimal design |
|
| Authors: | M. Claeys-Bruno O. Vandenabeele-Trambouze M. Sergent M. Geffard D. Bodet M. Dobrijevic A. Commeyras R. Phan Tan Luu |
| Affiliation: | aLaboratoire de Méthodologie de la Recherche Expérimentale, Université Paul Cézanne, Aix-Marseille III, faculté des sciences et techniques, avenue Escadrille Normandie-Niemen 13397, Marseille CEDEX 20, France bOrganisation Moléculaire, Evolution et Matériaux Fluorés, UMR-CNRS 5073, Université Montpellier II, 2 Place E. Bataillon, 34195 Montpellier CEDEX 5, France cENSCPB, PIOM, EPHE, 16, avenue Pey-Berland-33607 PESSAC Cedex, France dLaboratoire d'astrodynamique, d'astrophysique et d'aéronomie de Bordeaux, UMR-CNRS 5804, OASU, 2 rue de l'observatoire, BP 89, 33270 Floirac, France |
| Abstract: | Using derivatization by chloroethylnitrosourea (CENU) a new strategy was used for raising antibodies directed against hapten (< 300 Da) and the quantification of these haptens by ELISA. After raising antibodies directed against histamine, they were characterized and used for ELISA measurements. The development of a quantitative method needs an optimization of both detection and derivatization step. Experimental design methodology has been applied to optimize the conditions of the detection of the derivatized histamine. Methods and results were closely related. |
| Keywords: | Experimental design Hybrid design Antibodies Derivatization Histamine analysis |
| 本文献已被 ScienceDirect 等数据库收录！ | |