首页 | 本学科首页   官方微博 | 高级检索  
     

高纯度新疆胡麻卵磷脂的制备
引用本文:关明,杨光勇,库里松·哈衣尔别克,阿孜古丽·叶海亚.高纯度新疆胡麻卵磷脂的制备[J].食品科学,2007,28(9):176-180.
作者姓名:关明  杨光勇  库里松·哈衣尔别克  阿孜古丽·叶海亚
作者单位:新疆师范大学生命科学与化学学院; 新疆化学工业学校; 新疆师范大学生命科学与化学学院 新疆乌鲁木齐830054; 新疆乌鲁木齐830021; 新疆乌鲁木齐830054;
基金项目:新疆维吾尔自治区高校科研计划立项项目(XJEDU2006S29);新疆师范大学2005年校基金资助项目
摘    要:以粉末状新疆胡麻磷脂粗品为原料,经金属离子沉淀法、柱层析法、全溶剂萃取法及薄层层析富集法等工艺制备了高纯度卵磷脂,并研究了金属离子沉淀法对柱层析分离磷脂混合物效果的影响。结果表明:以金属离子锌为复合沉淀离子,以中性氧化铝为吸附剂,以氯仿:甲醇:水=65:25:4(V:V:V)为洗脱剂进行等度洗脱,可获得高纯度的卵磷脂精品;未经金属离子沉淀法处理的磷脂混合物,氧化铝吸附柱层析法无法分离得到单一组分的卵磷脂,对于该部分磷脂混合物,研究采用薄层层析富集法获得卵磷脂纯品。所得卵磷脂采用红外光谱、紫外光谱、一维多次展开薄层层析、二维双液展开薄层层析等手段进行了鉴定。

关 键 词:胡麻卵磷脂    磷脂酰胆碱    柱层析    薄层层析  
文章编号:1002-6630(2007)09-0176-05
修稿时间:2007-07-05

Manufacture of High Purity Xinjiang Flax Lecithin
GUAN Ming,YANG Guang-yong,KULISONG Hayierbieker,AZIGULI Yehaiya.Manufacture of High Purity Xinjiang Flax Lecithin[J].Food Science,2007,28(9):176-180.
Authors:GUAN Ming  YANG Guang-yong  KULISONG Hayierbieker  AZIGULI Yehaiya
Affiliation:1.College of Life and Environment Sciences,Xinjiang Normal University,Urumqi 830054,China;2.Xinjiang Chemistry and Industry School,Urumqi 830021,China
Abstract:This research take the powdery Xinjiang flax phosphatide as the raw material,obtains the high-purity lecithin after applying the methods of metallic ion precipitation method,column chromatographic analysis,entire solvent extraction method and thin layer chromatographic analysis.And it has studied the effect influence of metallic ion precipitation method on the separation of phosphatide mixture by the columns chromatographic analysis.The result indicated that,take the metallic ion zinc as the compound precipating ion,and the neutral aluminum oxide as the absorbent,take chloroform:methyl alcohol:water=65:25:4(V:V:V)to elutes the medicinal preparation,the high-purity lecithin can be obtained;the phospho-lipin mixture without the metallic ion precipitation is unable to separate the sole component lecithin,regarding this part of phospho-lipin mixture,the research uses the thin layer chromatographic analysis to obtain the pure lecithin.The obtained lecithin has been distinguisded by the infrared spectrum,the ultra-violet spectrograph,one-dimensional launches the thin layer chromatographic analysis,two-dimensional double fluid launches method and so on.
Keywords:flax lecithin  phosphatidyl choline  column chromatographic analysis  thin layer chromatographic analysis
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《食品科学》浏览原始摘要信息
点击此处可从《食品科学》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号