Production of arachidonic acid by filamentous fungus, Mortierella alliacea strain YN-15 |
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Authors: | Tsunehiro Aki Yumiko Nagahata Katsuyuki Ishihara Yoshio Tanaka Tsutomu Morinaga Kenichi Higashiyama Kengo Akimoto Shigeaki Fujikawa Seiji Kawamoto Seiko Shigeta Kazuhisa Ono Osamu Suzuki |
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Affiliation: | (1) Institute for Fundamental Research, Suntory Ltd., Shimamoto, 618-0001 Mishima, Osaka, Japan;(2) Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-4-1 Kagamiyama, 739-8527 Higashi-Hiroshima, Japan;(3) Present address: Sagami Chemical Research Center, Sagamihara, 229-0012 Kanagawa, Japan;(4) Present address: Faculty of Biological Resources, Hiroshima Prefectural University, 562 Shichizuka Shobara, Hiroshima 727-0023, Japan |
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Abstract: | A filamentous fungus producing significant levels of arachidonic acid (AA, C20∶4n−6) was isolated from a freshwater pond sample
and assigned to the species Mortierella alliacea. This strain, YN-15, accumulated AA mainly in the form of triglyceride in its mycelia. An optimized culture in 25 L of medium
containing 12% glucose and 3% yeast extract yielded 46.1 g/L dry cell weight, 19.5 g/L total fatty acid, and 7.1 g/L AA by
7-d cultivation in a 50-L jar fermenter. Assimilation of soluble starch by YN-15 was notably enhanced by the addition of oleic
acid, soybean oil, ammonium sulfate, or potassium phosphate to a starch-based medium. Using starch as a main carbon source
in the pre-pilot scale cultivation improved the production of AA by up to 5.0 g/L. Mortierella alliacea strain YN-15 is therefore a promising fungal isolate for industrial production of AA and other polyunsaturated fatty acids. |
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Keywords: | Arachidonic acid microbial lipid production Mortierella alliacea polyunsaturated fatty acid single-cell oil |
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