| 航天诱变黑曲霉菌株ZM-8产纤维素酶的固态发酵条件研究 |
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| 引用本文： | 马旭光,张宗舟,刘星斌.航天诱变黑曲霉菌株ZM-8产纤维素酶的固态发酵条件研究[J].中国酿造,2008(23). |
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| 作者姓名： | 马旭光 张宗舟 刘星斌 |
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| 作者单位： | 天水师范学院生命科学与化学学院;甘肃农业大学农学院; |
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| 基金项目： | 国家科技支撑计划项目(2007BAD89B17); 2007年天水师范学院院列重点项目(TSA0704) |
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| 摘 要： | 以麦秸、麸皮为原料,利用固态发酵对航天诱变后筛选的黑曲霉ZM-8菌株生产纤维素酶的条件进行了研究。结果表明,最佳培养基配方为:小麦秸秆粉与麸皮质量比为8:2,(NH4)2SO4为4%,料水比为1:2;最佳培养条件为:接种量为6%,初始pH值为6.5,培养温度为28℃,培养时间为96h。在以上的培养基和培养条件下,测定滤纸酶(FPA)、羧甲基纤维素酶(CMC)和β-葡萄糖苷酶的活力分别为7.90U/g、24.99U/g、21.74U/g,比出发菌种分别提高了3.0倍、1.66倍、2.24倍。
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| 关 键 词： | 黑曲霉 纤维素酶 固态发酵 |
Study on solid-state fermentation conditions of space mutated Aspergillus niger ZM-8 for cellulase production |
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| MA Xuguang,ZHANG Zongzhou,LIU Xingbin.Study on solid-state fermentation conditions of space mutated Aspergillus niger ZM-8 for cellulase production[J].China Brewing,2008(23). |
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| Authors: | MA Xuguang ZHANG Zongzhou LIU Xingbin |
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| Affiliation: | MA Xuguang1,ZHANG Zongzhou1,LIU Xingbin2 (1. College of Life Science , Chemistry,Tianshui Normal University,Tianshui 741001,China,2. College of Agriculture,Gansu Agricultural University,Lanzhou 730070,China) |
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| Abstract: | Using wheat stalk and wheat bran as the materials, the conditions for producing cellulase by solid fermentation of the space mutant strain Aspergillus niger ZM-8 were studied. The results indicated that the optimum medium for cellulase production were as follow: the mass ratio of wheat stalk to wheat bran was 8:2, 4% (NH4)2SO4 was used as nitrogen source, and the ratio of material to water was 1:2. The optimum culture conditions were as follows: the inoculum was 6%, the initial pH was 6.5, the culture tempe... |
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| Keywords: | Aspergillus niger cellulase solid fermentation |
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