| Abstract: | Arylamidase activity in human endometrium tissue, uterine fluid, and serum in uterine dysfunction induced by a copper IUD, steroidal contraception, and lactational amenorrhea was investigated. Arylamidase activity in cell-free preparation of endometrium increased steadily from the early proliferative phase attaining the maximum level at the secretory phase. The activity in the uterine fluid and serum showed similar trends. In uterine dysfunction, the activity decreased in different conditions. The distribution of the enzyme activity in the particular and soluble fractions was within a close range (1.4 to 1.7), indicating the absence of transfer of enzyme from particulate to the soluble fraction. The administration (in vitro) of 10 -4 M estrogen and progesterone caused an increase in the activity. Substrate affinity values of the enzymes in both fractions remained unaffected while the catalytic phase values were altered indicating a qualitative difference in the 2 enzymes. |
