Cellulolytic enzymes produced by a newly isolated soil fungus Penicillium sp. TG2 with potential for use in cellulosic ethanol production |
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| Affiliation: | 1. Biofinery Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Jeonbuk 580-185, South Korea;2. Department of Environmental Biotechnology, Chonbuk National University Iksan Campus, Ma-dong, Iksan-si, Jeonbuk 570-752, South Korea;1. Department of Electrical and Electronic Engineering, Ritsumeikan University, 1-1-1 Nojihigashi, Kusatsu, Shiga 525-8577, Japan;2. Environment and Energy Research Center, Nitto Denko Corporation, 2-8 Yamadaoka, Suita, Osaka 565-0871, Japan;1. HEP-Renewable Energy Sources Ltd., Ulica grada Vukovara 37, 10 000 Zagreb, Croatia;2. University of Zagreb, Faculty of Electrical Engineering and Computing, Unska 3, 10 000 Zagreb, Croatia;1. Department of Microbiology, Guru Nanak Dev University, Amritsar 143 005, India;2. Central Institute of Post Harvest Engineering Technology (CIPHET), Ludhiana 141 004, India;1. Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 737-0046, Japan;2. Department of Forest Biomaterials Engineering, College of Forest and Environmental Sciences, Kangwon National University, 192-1 Hyoja, Chuncheon 200-701, South Korea;3. Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6226, USA;4. BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6226, USA;1. International Institute for Applied Systems Analysis (IIASA), Austria;2. University of Hawaii at Manoa, Department of Urban and Regional Planning, USA;3. University of Hawaii Economic Research Organization, USA;4. University of Hawaii at Manoa, Department of Natural Resources and Environmental Management, USA |
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| Abstract: | A newly isolated soil fungus, Penicillium sp. TG2, had cellulase activities that were comparable to those of Trichoderma reesei RUT-C30, a common commercial strain used for cellulase production. The maximal and specific activities were 1.27 U/mL and 2.28 U/mg for endoglucanase, 0.31 U/mL and 0.56 U/mg for exoglucanase, 0.54 U/mL and 1.03 U/mg for β-glucosidase, and 0.45 U/mL and 0.81 U/mg for filter paper cellulase (FPase), respectively. Optimal FPase activity was at pH 5.0 and 50 °C. We used a simultaneous saccharification and fermentation (SSF) process, which employed the yeast Kluyveromyces marxianus and Penicillium sp. TG2 cellulolytic enzymes, to produce ethanol from empty palm fruit bunches (EFBs), a waste product from the palm oil industry. The present findings indicate that Penicillium sp. TG2 has great potential as an alternative source of enzymes for saccharification of lignocellulosic biomass. |
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| Keywords: | Cellulosic materials Cellulose Empty palm fruit bunches Ethanol Simultaneous saccharification and fermentation |
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