An optical biosensor-based immunoassay for the determination of bovine serum albumin in milk and milk products |
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| Affiliation: | 1. Fonterra Co-operative Group Ltd, Cnr No. 1 Road & SH 26, Waitoa, New Zealand;2. R J Hill Laboratories Ltd, Clyde St, Hamilton, New Zealand;1. Dipartimento di Chimica, Università di Pavia, Via Taramelli 12, 27100, Pavia, Italy;2. Departament de Química, Universitat de Lleida, Rovira Roure 191, 25198, Lleida, Spain;1. College of Science, Dalian Jiaotong University, Dalian 116028, PR China;2. School of Mathematics, Liaoning University, Shenyang 110036, PR China;1. Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Provincial Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai, Shandong 264003, PR China;2. University of Chinese Academy of Sciences, Beijing 100049, PR China;1. Division of Social Science, 600 E. 4th St., Morris, MN 56267, United States;2. University of Minnesota Morris, 600 E. 4th St., Morris, MN 56267, United States;1. Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA;2. Department of Materials Science and Engineering, University of Wisconsin-Madison, Madison, WI, USA;3. Department of Orthopedics and Rehabilitation, University of Wisconsin-Madison, Madison, WI, USA;1. Fakultät für Mathematik, Universität Bielefeld, 33501 Bielefeld, Germany;2. Charles University, Faculty of Mathematics and Physics, Mathematical Institute, Sokolovská 83, 186 75 Praha 8, Czech Republic |
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| Abstract: | An automated biosensor immunoassay, exploiting surface plasmon resonance detection, is described for the quantitation of bovine serum albumin (BSA) in milk products. Antibody selection, assay conditions and potential non-specific binding interferences were defined. Analytical performance includes a working range of 10–1000 ng mL−1, a method detection limit of 0.02 mg g−1 in milk, an instrument intermediate precision relative standard deviation (RSDiR) of 3.7%, an intermediate precision RSDiR of 8.9% for whey protein concentrate, and a single flow cell stable for at least 400 cycles. Accuracy was demonstrated by recovery, compliance with a certified reference material and comparison with a high performance liquid chromatographic method. The technique was applied to the estimation of BSA content of bovine milk, colostrum, whey protein fractions and infant formulae. The change in BSA expression during early bovine lactation and across a production season, and the thermal denaturation of BSA were also investigated. |
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