Improvement of enzymatic saccharification of Populus and switchgrass by combined pretreatment with steam and wet disk milling |
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| Affiliation: | 1. Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 737-0046, Japan;2. Department of Forest Biomaterials Engineering, College of Forest and Environmental Sciences, Kangwon National University, 192-1 Hyoja, Chuncheon 200-701, South Korea;3. Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6226, USA;4. BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6226, USA;1. Geological Survey of Japan, AIST, Tsukuba Central 7, 1-1-1 Higashi, Tsukuba 305-8567, Japan;2. Faculty of International Resource Sciences, Akita University, 1-1 Tegatagakuencho, Akita 010-8502, Japan;3. North Japan Research Institute for Sustainable Energy, Hirosaki University, 2-1-3 Matsubara, Aomori 030-0813, Japan;1. University of Belgrade, Institute of Nuclear Sciences ‘‘Vinca’’, Laboratory for Thermal Engineering and Energy, P.O. Box 522, 11001 Belgrade, Serbia;2. University of Belgrade, Faculty of Mechanical Engineering, Innovation Centre, Kraljice Marije 16, 11120 Belgrade 35, Serbia;3. University of Belgrade, Faculty of Mechanical Engineering, Kraljice Marije 16, 11120 Belgrade 35, Serbia;1. Department of Chemical Engineering, Faculty of Engineering, Mahidol University, Nakorn Pathom 73170, Thailand;2. Scientific Equipment and Research Division, Kasetsart University Research and Development Institute (KURDI), Kasetsart University, Bangkok 10900, Thailand;3. Department of Electrical Engineering, Faculty of Engineering, Mahidol University, Nakorn Pathom 73170, Thailand;1. Università degli Studi di Padova, Department of Land, Environment, Agriculture and Forestry, Viale dell’Università 16, 35020 Legnaro, Padova, Italy;2. Consiglio per la Ricerca e la sperimentazione in Agricoltura, Unità di ricerca per l''ingegneria agraria, Via della Pascolare 16 00015 Monterotondo Scalo, Rome, Italy;1. Sustainable Energy Technologies Department, Brookhaven National Laboratory, Upton, NY, USA;2. Department of Electrical and Electronics Engineering, Abdullah Gul University, Kayseri, Turkey;3. Electrical Distribution Design, Inc., Blacksburg, VA, USA;4. Department of Electrical and Computer Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA;5. Pepco Holdings, Inc., Washington, D.C., USA |
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| Abstract: | To reduce the recalcitrance of lignocellulosic biomass for subsequent biological processing, we pretreated energy crop feedstocks with mild steam treatment (ST; 130 and 150 °C for 60 min) and wet disk milling (WDM). We tested two phylogenetically different, but typical energy crop feedstocks: Populus trichocarpa and switchgrass (Panicum virgatum). WDM after ST facilitated the fibrillation of both types of biomass, resulting in an increase of specific surface area, improved enzymatic saccharification yield, and decrease in cellulose crystallinity. After steam treatment at 150 °C followed by 17 cycles of WDM, enzymatic hydrolysis resulted in almost complete glucan to glucose conversion in both feedstocks. |
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| Keywords: | Hot-compressed water Wet disk milling Switchgrass Biorefinery steam treatment" },{" #name" :" keyword" ," $" :{" id" :" kwrd0040" }," $$" :[{" #name" :" text" ," _" :" ST wet disk milling" },{" #name" :" keyword" ," $" :{" id" :" kwrd0050" }," $$" :[{" #name" :" text" ," _" :" WDM hot-compressed water treatment" },{" #name" :" keyword" ," $" :{" id" :" kwrd0060" }," $$" :[{" #name" :" text" ," _" :" HCWT high-performance liquid chromatography" },{" #name" :" keyword" ," $" :{" id" :" kwrd0070" }," $$" :[{" #name" :" text" ," _" :" HPLC specific surface area" },{" #name" :" keyword" ," $" :{" id" :" kwrd0080" }," $$" :[{" #name" :" text" ," _" :" SSA crystallinity index" },{" #name" :" keyword" ," $" :{" id" :" kwrd0090" }," $$" :[{" #name" :" text" ," _" :" CrI scanning electron microscope" },{" #name" :" keyword" ," $" :{" id" :" kwrd0100" }," $$" :[{" #name" :" text" ," _" :" SEM |
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