Quantification of Dendritic Spines Remodeling under Physiological Stimuli and in Pathological Conditions |
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Authors: | Ewa Bczyska Katarzyna Karolina Pels Subhadip Basu Jakub Wodarczyk Baej Ruszczycki |
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Affiliation: | 1.Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Street, 02-093 Warsaw, Poland; (E.B.); (K.K.P.); (J.W.);2.Department of Computer Science and Engineering, Jadvapur University, Kolkata 700032, India; |
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Abstract: | Numerous brain diseases are associated with abnormalities in morphology and density of dendritic spines, small membranous protrusions whose structural geometry correlates with the strength of synaptic connections. Thus, the quantitative analysis of dendritic spines remodeling in microscopic images is one of the key elements towards understanding mechanisms of structural neuronal plasticity and bases of brain pathology. In the following article, we review experimental approaches designed to assess quantitative features of dendritic spines under physiological stimuli and in pathological conditions. We compare various methodological pipelines of biological models, sample preparation, data analysis, image acquisition, sample size, and statistical analysis. The methodology and results of relevant experiments are systematically summarized in a tabular form. In particular, we focus on quantitative data regarding the number of animals, cells, dendritic spines, types of studied parameters, size of observed changes, and their statistical significance. |
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Keywords: | dendritic spines dendritic spine analysis spine remodeling synaptic plasticity structural plasticity neuronal remodeling dendritic spine morphology |
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