| 沙棘籽粕生物碱的提取分离及对乳鼠心肌细胞损伤的保护作用 |
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| 引用本文： | 胡长鹰,徐德平. 沙棘籽粕生物碱的提取分离及对乳鼠心肌细胞损伤的保护作用[J]. 食品科学, 2010, 31(9): 234-237. DOI: 10.7506/spkx1002-6630-201009052 |
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| 作者姓名： | 胡长鹰 徐德平 |
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| 作者单位： | 1.暨南大学食品科学与工程系 2.江南大学食品学院 |
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| 基金项目： | “十一五”国家科技支撑计划项目(2007BAD83B02) |
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| 摘 要： | 目的：研究沙棘籽粕中生物碱的提取分离及其对乳鼠心肌细胞缺血/ 再灌注损伤的保护作用。方法：用乙醇提取、利用多种柱层析分离手段从沙棘籽粕中分离单体化合物，采用质谱、核磁共振法鉴定其结构；采用乳鼠心肌细胞缺血/ 再灌注损伤模型，利用MTT 法测定心肌细胞存活率，检测乳酸脱氢酶(LDH)、肌酸激酶(CK)含量以反映心肌细胞受损的程度。结果：从沙棘籽粕分离得到SJ-A- Ⅰ组分，经鉴定为生物碱5,11- 二羟基色胺；该物质能有效维持心肌细胞活力，减少培养心肌细胞缺血/ 再灌注损伤所致心肌酶(LDH、CK)的释放。结论：沙棘籽粕中的生物碱5,11-二羟基色胺对乳鼠心肌细胞缺血/再灌注损伤具有明显的保护作用，其最佳质量浓度为150mg/L。
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| 关 键 词： | 沙棘籽粕 生物碱 5 11- 二羟基色胺 心肌细胞 缺血/ 再灌注 |
| 收稿时间： | 2009-09-21 |
Extraction,Isolation and Protective Effect of Alkaloid from Seabuckthorn Seeds on Injured Cardiomyocytes in Rats |
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| HU Chang-ying,XU De-ping. Extraction,Isolation and Protective Effect of Alkaloid from Seabuckthorn Seeds on Injured Cardiomyocytes in Rats[J]. Food Science, 2010, 31(9): 234-237. DOI: 10.7506/spkx1002-6630-201009052 |
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| Authors: | HU Chang-ying XU De-ping |
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| Affiliation: | 1. Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China ；2. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China |
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| Abstract: | Objective:To investigate the protective effect of alkaloid from seabuckthorn seeds on injured cardiomyocytes in rats.Methods:EtOH extract from seabuckthorn seeds was purified by column chromatography and its structure was elucidated by MS and NMR spectroscopic analyses.A model of cardiac ischemic/reperfusion(I/R) in vitro was established using the primary cultured rat cardiomyocytes.The viability of cardiomyocytes was evaluated by MTT assay and the contents of lactate dehydrogenase(LDH) and creatine kinase(... |
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| Keywords: | seabuckthorn seed alkaloid 5 11-dihydroxyltryptamine cardiomyocyte ischemia/reperfusion |
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