EFFECTS OF FOUR SPECIES OF BACTERIA ON PORCINE MUSCLE 2. Electrophoretic Patterns of Extracts of Salt-Soluble Protein |
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Authors: | R. J. BORTON L. J. BRATZLER J. F. PRICE |
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Affiliation: | Department of Food Science, Michigan State University, East Lansing, Michigan 48823 |
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Abstract: | SUMMARY– Electrophoresis of 0.6 M KCI extracts of porcine longissimus dorsi muscle revealed little change in the type or number of protein bands found either by starch-urea or disc-urea gel electrophoresis. The 0.6 M KCI extracts of muscle samples inoculated with Pediococcus cerevisiae, Leuconostoc mesenteroides and Micrococcus luteus and stored at 2 and 10°C for 20 days did not differ electrophoretically from control samples. Extracts of samples inoculated with Pseudomonas fragi showed a loss in the number of protein bands on starch-urea gel and disc-urea gel electrophoresis, indicating this organism exhibited some proteolytic effect upon the myofibrillar proteins . |
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