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EFFECTS OF FOUR SPECIES OF BACTERIA ON PORCINE MUSCLE 2. Electrophoretic Patterns of Extracts of Salt-Soluble Protein
Authors:R. J. BORTON  L. J. BRATZLER  J. F. PRICE
Affiliation:Department of Food Science, Michigan State University, East Lansing, Michigan 48823
Abstract:SUMMARY– Electrophoresis of 0.6 M KCI extracts of porcine longissimus dorsi muscle revealed little change in the type or number of protein bands found either by starch-urea or disc-urea gel electrophoresis. The 0.6 M KCI extracts of muscle samples inoculated with Pediococcus cerevisiae, Leuconostoc mesenteroides and Micrococcus luteus and stored at 2 and 10°C for 20 days did not differ electrophoretically from control samples. Extracts of samples inoculated with Pseudomonas fragi showed a loss in the number of protein bands on starch-urea gel and disc-urea gel electrophoresis, indicating this organism exhibited some proteolytic effect upon the myofibrillar proteins .
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