高效液相色谱法测定维生素片中维生素B12的不确定度评定

目的评定高效液相色谱法测定维生素片中维生素B_(12)的不确定度。方法维生素片中维生素B_(12)经纯化水超声提取,经色谱柱分离,流动相等度洗脱,使用紫外检测器550 nm检测,外标法定量来测定维生素片中维生素B_(12)的含量。依据JJF 1059.1-2012《测量不确定度评定与表示》,考察称量、标准溶液配制、样品和仪器重复测量等主要因素引入的不确定度,并对不确定度的各分量进行计算和合成。结果当维生素片中维生素B_(12)含量为0.408 mg/100 g时,在95%的置信区间下,其扩展不确定度为±0.0053 mg/100 g(k=2)。评定结果表明,实验过程中的不确定度主要来源于标准溶液配制、仪器重复测量。结论不确定度评定适用于高效液相色谱法快速测定维生素片中维生素B_(12)含量的不确定度分析,对检测结果准确度的提高具有指导意义。

Determination of vitamin B12 in vitamin tablets by high performance liquid chromatography

Objective Determination of vitamin B12 in vitamin tablets by high performance liquid chromatography. Methods Vitamins (vitamin B12 by purified water ultrasonic extraction, the chromatographic column separation and elution flow equal degrees, use 550 nm uv detector to detect, external standard method for quantitative determination of the content of vitamins in the vitamin B12. According to JJF 1059.1-2012 "measurement uncertainty evaluation and said", weighing, standard solution preparation, sample and repeated measurement instrument main factors such as the introduction of uncertainty, and a calculation is made for each component of uncertainty and synthesis. Results When the vitamin B12 content was 0.42 mg/100g, the expansion uncertainty was positive and negative 0.0054mg/100g(k=2) in 95% confidence interval. The evaluation results show that the uncertainty of the experiment is mainly derived from the standard solution and the repeated measurement of the instrument. .Conclusion The uncertainty evaluation is applicable to the rapid determination of vitamin B12 content in vitamin tablets by high performance liquid chromatography (HPLC), which is of guiding significance to the improvement of accuracy of test results.