银纳米粒子基底制备及表面增强拉曼光谱法检测荧光素钠

目的制备具有增强效应的银纳米粒子作为表面增强拉曼光谱的基底,应用于荧光素钠色素的检测,提供一种快速、方便、超灵敏的检测手段。方法用水热法制备银纳米粒子作为表面增强拉曼光谱的基底,通过紫外可见吸收光谱研究以及对比不同激发波长作用下的银纳米粒子增强效应,优化实验条件,对不同浓度的荧光素钠色素进行检测。结果银纳米粒子基底展现出了超灵敏的检测限度和超高的增强效应,在激发波长514 nm、激光强度1 mw、收集时间5 s的实验条件下,荧光素钠色素分子的检测限度达3.01×10~(-4) mg/kg。结论本方法制备的银纳米材料可作为表面增强拉曼光谱的基底,有效应用于荧光素钠色素的检测中,在进口食品安全监测中具有重要的应用价值。

Preparation of Ag nanoparticale substrate and determination of sodium fluorescein by surface enhanced Raman spectroscopy

Objective: To rapid and ultrasensitive detecting on the sodium fluorescin by surface enhanced Raman spectra (SERS) technology through preparing SERS-active Ag nanoparticle. Methods: Ag nanoparticle was fabricated by hydrothermal method, served as the SERS substrate in situ detecting sodium fluorescein molecule at different concentrations. We discovered the enhancement mechanism of Ag nanoparticle platform with the help of UV-Visible absorption spectrum. Results: Ag nanoparticle exhibited an outstanding SERS performance and appeared better plasmon coupling effect in the wavenumber of 514nm with the limit of detection as low as 3.01 × 10-4 mg/kg and ultrahigh enhancement factor for sodium fluorescein target molecule. The optimal condition for the experiment are as follows, laser wavelength: 514 nm; power: 1 mw; acquisition time: 5s. Conclusion: We prepared Ag nanoparticle and made it as SERS substrate, which has great potential in imported food security because of its ultrahigh SERS performance, and contributing to human health.