| Abstract: | The glucose oxidase/peroxidase technique for determining microgram quantities of glucose in aqueous solution has been critically examined. The function of each of the constituents in the solution has been studied, and the relation between time and temperature of incubation determined. Potential errors due to the temperature-dependent pH-behaviour of tris buffers, and the presence in the solution to be analyzed of high concentrations of buffer salts, glycerol, and reducing substances, are discussed. The relation between the absorbance, and the type and normality of acid used to stabilize the colour is examined. |
