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Analysis of lichen thin sections by PIXE and STIM using a proton microprobe
Authors:B. M. Clark   N. F. Mangelson   L. L. St. Clair   J. S. Gardner   L. S. Cooper   L. B. Rees   P. G. Grant  G. S. Bench
Affiliation:

a Department of Chemistry and Biochemistry, Brigham Young University, C100 BNSN, Provo UT 84602-5700, USA

b Department of Botany and Range Science, Brigham Young University, Provo UT 84602, USA

c Department of Physics and Astronomy, Brigham Young University, Provo UT 84602, USA

d Center for Accelerator Mass Spectrometry, Lawrence Livermore National Laboratory, Livermore CA 94550, USA

Abstract:In order to better understand the distribution pattern of mineral elements in lichen tissues, thin sections (15 μm) of the foliose, vagrant soil lichen Xanthoparmelia chlorochroa were examined using proton microprobe Particle induced X-ray emission (PIXE). This technique was used to make two-dimensional scans, with 5 μm resolution, across tissue cross sections of the test species. Element maps for Si, P, S, Cl, K, Ca, Ti, Mn, Fe, Cu, Zn, and As have been prepared. Several elements are strongly localized in the element maps. PIXE data are complimented with STIM, light micrographs, and SEM images. Preliminary data suggest that nuclear microprobe techniques may be useful in elucidating element absorption and transport mechanisms in lichens.
Keywords:Proton microprobe   PIXE   Lichen   Element distribution
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