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层析填料中顽固色素去除的新方法研究
引用本文:翁志兵,王鹏红,赵力,夏松,金坚,朱祯平,李华钟. 层析填料中顽固色素去除的新方法研究[J]. 食品与生物技术学报, 2022, 41(2): 106-111
作者姓名:翁志兵  王鹏红  赵力  夏松  金坚  朱祯平  李华钟
作者单位:江南大学 生物工程学院,江苏 无锡 214122; 三生国健药业(上海)股份有限公司,上海 201210
摘    要:对纯化工艺中因铁离子污染形成黄褐色的阴离子填料进行了研究.该色素在碱性条件下产生并加重,利用常规方法高盐、表面活性剂均无法清除.作者使用磷酸、醋酸、柠檬酸、盐酸进行测试,发现浓磷酸去除填料色素的能力高于柠檬酸,而浓醋酸和浓盐酸不能明显去除填料色素.基于上述结论,进一步研究磷酸在不同浓度和不同处理时间下对填料中色素的影响...

关 键 词:阴离子填料  色素  抗体  技术方法  铁离子

A New Method of Removing Stubborn Pigments from Chromatographic Resin
WENG Zhibing,WANG Penghong,ZHAO Li,XIA Song,JIAN Jin,ZHU Zhenping,LI Huazhong. A New Method of Removing Stubborn Pigments from Chromatographic Resin[J]. Journal of Food Science and Biotechnology, 2022, 41(2): 106-111
Authors:WENG Zhibing  WANG Penghong  ZHAO Li  XIA Song  JIAN Jin  ZHU Zhenping  LI Huazhong
Affiliation:School of Biotechnology, Jiangnan University, Wuxi 214122, China; Sunshine GuoJian Pharmaceutical (Shanghai) Co., Ltd, Shanghai 201210, China
Abstract:This study investigated the yellow-brown anion exchange resin formed by ferric ion pollution in the purification process. The pigment is produced and aggravated under alkaline conditions, and cannot be removed by conventional methods such as high salt and surfactants. Phosphoric acid, acetic acid, citric acid and hydrochloric acid were used for testing in this study. It was found that concentrated phosphoric acid could remove resin pigments more effectively than citric acid, while concentrated acetic acid and hydrochloric acid could not. Based on the above conclusions, the effect of phosphoric acid on the pigment in the resin under different concentrations and treatment times was further studied. The results showed that the ferric ion concentration and resin capacity increased with the increase of phosphoric acid concentration, however, prolonging incubation time led to the increase ofresin capacity, but gradually decreased ferric ion concentration. Hence, it was inferred that during the treatment ofresin pigment with phosphoric acid, there was secondary adsorption of iron ions to the porous structure of resin lumen with the increase of incubation time, resulting in the gradual decrease of ferric ions in the solution. Through data analysis in combination with color contrast, it was determined that incubating anion exchange resins with 0.5 mol/L phosphoric acid for 8.75~11.35 minutes could significantly remove the stubborn pigment problems caused by ferric ions, and restored the resin capacity recover, while avoiding related impurities to pollute the final protein product. The new method for removing impurities in chromatography resin studied in this paper could provide a theoretical reference for the cost reduction and efficiency enhancement of related biological products in the downstream industrialization.
Keywords:anion exchange resin   pigment   antibody   technical method   Fe3+
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