抗耐甲氧西林金黄色葡萄球菌菌株的筛选与鉴定

通过对246株芽孢杆菌进行筛选，以期获得对耐甲氧西林金黄色葡萄球菌（MRSA）具抑制作用的菌株，抑菌结果表明，对耐甲氧西林金黄色葡萄球菌有抑制作用的菌株有5株，其中菌株1012-4的抑菌活性最强；为确定菌株种属，对优选菌株进行形态特征观察、生理生化特征鉴定以及API50 CHB鉴定，初步确定1012-4为枯草芽孢杆菌；为进一步确证菌株类型，实验采用了16S rDNA基因测序，序列结果表明，菌株1012-4为芽孢杆菌属枯草芽孢杆菌种；经反复确证，确定菌株1012-4为优势菌株，抗菌活性显著，为新型抗MRSA药物的研发提供了菌株来源。

Screening and Identification of Strains with Anti-methicillin-resistant Staphylococcus aureus Activity

In this research, strains with antibacterial activity on Methicillin-resistant Staphylococcus aureus.246 tested strains was screened and identified, Antibacterial test results showed that five strains had antibacterial activity, among which strain 1012-4 showed the strongest activity. Morphological, physiological and biochemical characterization, as well as API50 CHB method, were adopted to determine the type of strain 1012-4. Preliminary results suggested that the stain 1012-4 was of Bacillus subtilis species. Through 16S rDNA gene sequence alignment, the strain 1012-4 was further identified as the genus Bacillus and Bacillus subtilis species. Verification test confirmed that, the strong antibacterial activity of strain 1012-4. This research provided new strain source for the research and development of new anti-MRSA drugs.