Development of a new biosensor for mediatorless voltammetric determination of hydrogen peroxide and its application in milk samples |
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| Authors: | ?enol Alpat Sibel K?l?nç Alpat Zekerya Dursun Azmi Telefoncu |
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| Affiliation: | (1) Department of Chemistry Education, Dokuz Eylül University, Buca-Izmir, Turkey;(2) Department of Chemistry, Ege University, Bornova-Izmir, Turkey;(3) Department of Biochemistry, Ege University, Bornova-Izmir, Turkey |
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| Abstract: | A new biosensor for the voltammetric detection of hydrogen peroxide was developed based on immobilization of catalase on a
clinoptilolite modified carbon paste electrode using bovine serum albumin and glutaraldehyde. The biosensor response was evaluated
according to electrode composition, reaction time, solution pH and temperature. The voltammetric signals were linearly in
proportion to H2O2 concentration in the range 5.0 × 10−6–1.0 × 10−3 M with a correlation coefficient of 0.9975. The detection limit is 8.0 × 10−7 M and the relative standard deviation for 4.0 × 10−4 M hydrogen peroxide was 1.83% (n = 6). The biosensor exhibited high sensitivity, and it was determined that it could be used for more than 2 months. In addition,
the biosensor was successfully applied for the determination of hydrogen peroxide in milk samples. |
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| Keywords: | Hydrogen peroxide Voltammetry Biosensor Clinoptilolite Catalase Milk |
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