乳酸钠对铜绿假单胞菌生长的影响

为探讨乳酸钠对铜绿假单胞菌(Pseudomonas aeruginosa)的特定抑制作用，揭示乳酸钠质量浓度与介质pH值水平影响乳酸钠抑菌效果的规律，以营养肉汤培养基为介质，按照乳酸钠质量浓度(0、0.2、0.5、1.0、1.5、2.0g/100mL)单因素试验及乳酸质量浓度与介质pH值(4.0～6.2)的两因素交互试验设计，25℃恒温培养。结果表明：在适当的pH值范围内，乳酸钠(NaL)表现出良好的抑菌效果：当介质pH值小于NaL质量浓度的抑菌临界pH值时，NaL抑菌效果与其质量浓度成正比，介质pH4.8，抑菌效果2.0g/100mL NaL＞1.0g/100mL NaL＞0.5g/100mL NaL。NaL质量浓度一定，其抑菌效果与介质pH值成反比。

Effect of Sodium Lactate on the Growth of Pseudomonas aeruginosa

Nutrient broth media containing different concentrations of sodium lactate(0,0.2,0.5,1.0,1.5 g/100 mL and 2.0 g/100 mL;consequent pH 4.0-6.2) were used to culture Pseudomonas aeruginosa constantly at 25 ℃ to examine the inhibitory effect of sodium lactate on bacterial growth.In the proper pH range,sodium lactate showed outstanding antibacterial activity.At a medium pH level lower than the critical inhibitory pH,the antibacterial effect of sodium lactate was directly proportional to the corresponding concentration.At a medium pH of 4.8,sodium lactate exhibited the highest antibacterial activity at 2.0 g/100 mL,followed by 1.0 g/100 mL;the antibacterial activity was the weakest at 0.5 g/100 mL.At a constant sodium lactate concentration,the antibacterial activity was inversely proportional to medium pH.