携带socs3基因的溶瘤腺病毒的构建及体外抗肿瘤活性的研究

SOCS3（suppressor of cytokine signaling 3）是JAK/STAT信号通路的负调控因子,SOCS3间接调控的STAT3磷酸化与肿瘤的发生密切相关。本试验构建携带socs3的溶瘤腺病毒AdCN305-SOCS3,在细胞水平研究socs3对肿瘤的抑制作用,并评估AdCN305-SOCS3的抗肿瘤效果。该病毒感染多种肿瘤细胞,均能特异性扩增,同时表达功能正常的SOCS3蛋白,从而抑制STAT3磷酸化,引起肿瘤细胞的明显凋亡。对AdCN305-SOCS3的研究,证实SOCS3蛋白和AdCN305-SOCS3病毒都具有抗肿瘤的功能,在肿瘤的靶向基因治疗研究中具有广阔的应用前景。

Construction of the Oncolytic Adenovirus Carrying Human socs3 Gene and Investigation of Its Anti-tumor Activity in Vitro

SOCS3 is a suppressor of JAK/STAT signal transduction pathway and indirectly regulates STAT3 phosphorylation, which associates closely with the occurrence of cancer. Herein the authors con- struct an oncolytic adenoviral vector bearing socs3, termed as AdCN305-SOCS3, for the purpose of studying the suppressive effect of socs3 gene on tumor cells and evaluating anti-tumor dfficiency of AdCN305- SOCS3 in vitro specific replicated in various cancer cell lines. Meanwhile, functional SOCS3 protein is expressed with the data showing repressed STAT3 phosphorylation and remarkably induces tumor cell apoptosis in consequences. Our research on AdCN305-SOCS3 proves that both SOCS3 and AdCN305-SOCS3 have anti-tumor activity and hold great perspective in the research of cancer gene therapy.