复合乳酸菌改善小鼠肠道菌群功能的研究

作者探讨了包含动物双歧杆菌乳亚种XLTG11、植物乳植杆菌CCFM8661、副干酪乳酪杆菌Glory LP16和鼠李糖乳酪杆菌Glory LG12的复合乳酸菌粉对小鼠肠道菌群的影响。将48只BALB/c小鼠适应性喂养7 d后，随机分成阴性对照组、复合乳酸菌低剂量组、复合乳酸菌中剂量组、复合乳酸菌高剂量组，其中阴性对照组小鼠灌胃等体积的生理盐水，受试组每只分别灌胃5×10⁶、5×10⁷、5×10⁸CFU的复合乳酸菌粉。灌胃14 d后，测定小鼠体质量、肠道菌群结构、组织病理变化、肠道屏障功能、肠道通透性和短链脂肪酸(SCFAs)浓度。结果表明，与对照组相比，复合乳酸菌喂养可以显著提高小鼠体质量；粪便中乳杆菌、双歧杆菌等有益菌的数量显著增加，产气荚膜梭菌的数量显著下降；SCFAs的浓度明显提高，并伴随着LPS和D-乳糖的显著下降。总体来说，复合乳酸菌粉对调节小鼠肠道菌群具有显著改善作用。

Improvement of Intestinal Microbiota Function in Mice by Compound Lactic Acid Bacteria

The study investigated the effects of a compound lactic acid bacteria powder containing Bifidobacterium animalis subsp. lactis XLTG11, Lactobacillus plantarum CCFM8661, 文字Lactobacillus paracasei Glory LP16, and Lactobacillus rhamnosus Glory LG12 on the intestinal microbiota of mice. After adaptive feeding for 7 d, 48 BALB/c mice were randomly divided into negative control, low-, middle-, and high-dose of compound lactic acid bacteria groups. The negative control group received an equal volume of physiological saline via gavage, while the test groups received 5×10⁶, 5×10⁷, and 5×10⁸ CFU of the compound lactic acid bacteria powder, respectively. After 14 d of gavage, the body weight, intestinal microbiota, histopathology, intestinal barrier, intestinal permeability, and short-chain fatty acids (SCFAs) concentration of mice were determined. The results showed that compared with the control group, the feeding of compound lactic acid bacteria significantly increased the body weight of mice and the number of beneficial bacteria such as Lactobacillus and Bifidobacterium in the feces, while significantly decreased the number of Clostridium perfringens. The concentration of short-chain fatty acids increased significantly, accompanied by a significant decrease in LPS and D-lactose. Overall, the compound lactic acid bacteria powder demonstrated the significant improvements in regulating the intestinal microbiota of mice.