植物乳植杆菌CCFM8661缓解重金属铅诱导小鼠肝肾损伤的剂量效应

铅是一种常见的环境和工业毒物，可以对人体的神经系统、肝脏和肾脏等产生持久性的损伤，寻找有效的缓解策略是当前亟待解决的重要问题之一。为评价植物乳植杆菌CCFM8661对重金属铅诱导的小鼠肝肾损伤的剂量效应，探讨了不同剂量植物乳植杆菌CCFM8661对小鼠组织生理生化指标、菌群结构以及粪便代谢产物的影响。结果发现，重金属铅可以对小鼠肝脏、肾脏等组织造成显著损伤，主要表现为肝肾组织过氧化氢酶（CAT）活力、谷胱甘肽酶（GSH）活力显著降低，肠道菌群及其代谢产物组成紊乱。给小鼠口服植物乳植杆菌CCFM8661可以显著缓解铅对小鼠的这些影响，既上调了CAT、GSH的酶活力，缓解了肝肾的病理指标，同时显著上调肠道乳杆菌的相对丰度及异丁酸等产物的含量。这些结果说明，植物乳植杆菌可能通过调控肠道菌群及其代谢产物发挥缓解铅中毒的作用。值得注意的是，植物乳植杆菌CCFM8661对铅损伤的缓解作用表现出了显著的剂量效应，选择合适的摄入剂量是未来植物乳植杆菌CCFM8661应用和推广的重要前提。

Dose Effect of Lactiplantibacillus plantarum CCFM8661 in Alleviating Heavy Metal Lead-Induced Liver and Kidney Injury in Mice

Lead is a common environmental and industrial toxin, which can cause persistent damage to the human nervous system, liver, and kidney. Finding effective mitigation strategies is one of the urgent issues to address. To evaluate the dose-effect of Lactiplantibacillus plantarum CCFM8661 on liver and kidney injury induced by heavy metal lead in mice, the changes of tissue physiological and biochemical indexes, microbial structure, and fecal metabolites in mice treated with different doses of L. plantarum CCFM8661 were investigated. The results showed that the heavy metal lead could significantly damage the liver, kidney, and other tissues of mice, mainly manifested as the significant reduction in enzyme activities of catalase (CAT) and glutathione (GSH), and the disturbance of gut microbiota and its metabolites composition. Oral administration of L. plantarum CCFM8661 significantly alleviated these changes induced by lead in mice, not only up-regulating the enzyme activities of CAT and GSH, but also alleviating the pathological indicators of liver and kidney, and significantly up-regulating the relative abundance of intestinal Lactobacillus and the content of isobutyric acid and other products. These results suggest that L. plantarum may play a role in alleviating lead poisoning by regulating gut microbiota and its metabolites. Notably, L. plantarum CCFM8661 showed a significant dose effect on the mitigation of lead injury, making it essential to determine the appropriate intake dose for the future application and promotion of L. plantarum CCFM8661.