Green ultra-high pressure extraction of bioactive compounds from Haematococcus pluvialis and Porphyridium cruentum microalgae |
| |
| Affiliation: | 1. Departamento de Engenharia Química e Engenharia de Alimentos, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil;2. Departamento de Ciência e Tecnologia de Alimentos, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil;3. Departamento de Engenharia de Alimentos, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil;4. Laboratório de Cultivo de Algas (LCA), Departamento de Aquicultura, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil;1. Department of Chemical and Bioprocess Engineering, Pontificia Universidad Católica (UC) de Chile, Avda. Vicuña Mackenna 4860, Macul, Santiago, Chile;2. Laboratory of Foodomics, Bioactivity and Food Analysis Department, Institute of Food Science Research (CIAL-CSIC), Nicolás Cabrera 9, Campus UAM Cantoblanco, 28049 Madrid, Spain;1. Bio-Energy Research Center, Chonnam National University, Gwangju 61186, Republic of Korea;2. Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 61186, Republic of Korea;1. Department of Chemistry, Umeå University, 90187 Umeå, Sweden;2. Food Technology, Facultad de Veterinaria, Instituto Agroalimentario de Aragón-IA2 (Universidad de Zaragoza-CITA), Zaragoza, Spain;1. College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China;2. Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China;3. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China;4. Department of Oceanography, National Sun Yat-sen University, Kaohsiung 804, Taiwan;5. Asia-Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung 804, Taiwan;6. University Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan;7. Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan;8. Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan 701, Taiwan;9. Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen 361005, China |
| |
| Abstract: | Microalgae are considered prolific sources of bioactive compounds that can be useful for nutraceuticals. In this study, the potential of ultra-high pressure extraction (UHPE) for the simultaneous cell disruption and extraction of bioactives from two microalgae species, Haematococcus pluvialis and Porphyridium cruentum, was evaluated. The variables studied to extract carotenoids for H. pluvialis were pressure (100–600 MPa) and number of cycles (1 and 3 cycles) whereas the variables studied to obtain bioactives such as B-phycoerythrin, carotenoids, and PUFAs for P. cruentum were pressure (100–600 MPa) and different extraction solvents (water, ethanol, ethyl acetate or ethanol/d-limonene), generally recognized as safe (GRAS). The UHPE results showed significant increase on the extraction of carotenoids (109.74–119.34 mg per g extract) from H. pluvialis using 1 cycle of 20 min regardless of the pressure used. For P. cruentum, an UHPE with water provided extracts enriched in B-phycoerythrin (up to 144.43 mg per g extract), while subsequent UHPE using ethanol, ethyl acetate or ethanol/d-limonene 1:1 (v/v) provided extracts enriched in carotenoids (up to 65.05 mg per g extract) and polyunsaturated fatty acids (mainly eicosapentanoic acid and linoleic acid). Therefore, UHPE proved to be a viable green alternative for the recovery of bioactives from microalgae biomass.Industrial relevance: Microalgae are promising sources of bioactives such as B-phycoerythrin, carotenoids and polyunsaturated fatty acids. The potential of ultra-high-pressure extraction (UHPE) has been demonstrated as a fast and viable eco-friendly alternative using GRAS solvents (water, ethanol, ethyl acetate or ethanol/d-limonene) for the simultaneous cell disruption and extraction of these bioactives from Haematococcus pluvialis and Porphyridium cruentum. The bioactives obtained with one step or two step-UHPE process can be used in an array of food, cosmetic and pharmaceutical applications. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
