三种龙葵果提取物的体外抗氧化及抗炎活性评价

龙葵果被广泛用于解热和利尿。本文研究了黑果龙葵(SNL)、黄果龙葵(SDL)和红果龙葵(SAM)三种龙葵果的石油醚(PE)、乙酸乙酯(Eto Ac)、正丁醇(n-Bu OH)萃取物体外抗氧化和抗炎能力。分别以DPPH·清除试验和二价铁离子还原能力(FRAP)探讨了体外抗氧化能力,以抗坏血酸(Vc)作为阳性对照;并以透明质酸酶和脂氧合酶抑制能力评价其抗炎效果,分别以双氯芬酸钠和去甲二氢愈创木酚作为阳性对照。三种龙葵果不同溶剂萃取物中,黑果龙葵乙酸乙酯萃取物抗氧化能力最强,DPPH·清除试验IC50为119.43μg/mL,FRAP为2.674 mM FeSO4/L;红果石油醚萃取物抗炎效果最好,透明质酸酶和脂氧合酶抑制IC50分别为810.67μg/mL和781.28μg/mL。黑果龙葵中总酚含量最高为27.16 mg/g。红果龙葵中黄酮含量最高为18.59 mg/g。这一研究为天然抗氧化剂和抗炎剂的开发提供了理论依据。

Evaluation on Antioxidant and Anti-inflammation in Vitro of Extracts of Three Solanum Nigrum L. Berries

Solanum nigrum L. berries have been widely used for anti-pyretic and diuretic in China. Antioxidant and anti-inflammation activities in vitro of extracts of Solanum nigrum Linnaeus(SNL), Solanum diphyllum Linnaeus(SDL) and Solanum alatum Moench(SAM) berry which were prepared by petroleum ether(PE), ethyl acetate(EtoAc) and n-butanol(n-BuOH). The antioxidant activites were evaluated by FRAP and DPPH· scavenging assays. Vitamin C was used as positive control. The results showed that DPPH· scavenging IC50 and FRAP values of SNL-Eto Ac extract were 119.43±1.19 μg/mL and 2.674±0.071 m M Fe SO4/L, respectively, indicating that it had the highest antioxidant activity. The anti-inflammation effect of different extracts was determined by hyaluronidase and lipoxygenase inhibition. SAM-PE extract had the highest anti-inflammation activity, which IC50 were 810.67±79.02 and 781.28±28.01 μg/ml, respectively. Diclofenac sodium and 4,4’-(2,3-Dimethyltetramethylene) dipyrocatechol were used as anti-inflammatory positive control. SNL had the highest content of total phenols(27.16±0.14 mg/g). SAM had the highest content of total flavonoids(18.59±0.14 mg/g). The results provided a theoretical supporting for developing natural antioxidant and anti-inflammatory resources.