Effect of Peroxiredoxin 1 on the biological function of airway epithelial cells and epithelial-mesenchymal transition |
| |
Authors: | HUOGEN LIU YUNDI SHI XIN WAN YING LIU HAILIN SHU FENGMING HUANG ZHENBIN GONG LING GU |
| |
Affiliation: | Department of Critical Care Medicine, Mindong Hospital Affiliated to Fujian Medical University, Ningde, 355000, China |
| |
Abstract: | Peroxiredoxin 1 (PRDX1) participates in tumor cell proliferation, apoptosis, migration, invasion, and the epithelial-to-mesenchymal transition (EMT). This study aimed to investigate the effect of PRDX1 on the EMT of airway epithelial cells stimulated with lipopolysaccharide (LPS) and transforming growth factor-beta 1 (TGF-β1). PRDX1 overexpression significantly increased the proliferation and migration of human bronchial epithelial (BEAS-2B) cells, reduced cell apoptosis (p < 0.01), and induced EMT and collagen deposition by upregulating the expression of the matrix metallopeptidase (MMP)2, MMP9, α-smooth muscle actin (α-SMA), N-cadherin, vimentin and twist proteins and inhibiting E-cadherin expression (p < 0.05). PRDX1 overexpression promoted TGF-β1-mediated inhibition of cell proliferation and migration and significantly enhanced the TGF-β1-induced EMT and collagen synthesis (p < 0.05). Knockdown of PRDX1 inhibited cell proliferation, migration, EMT, and collagen synthesis (p < 0.01), reversed LPS-mediated inhibition of cell proliferation and migration, and significantly suppressed LPS-induced EMT and collagen synthesis (p < 0.01). The result indicating that PRDX1 may be involved in LPS/TGF-1-induced EMT and collagen synthesis in human bronchial epithelial cells. |
| |
Keywords: | Peroxiredoxin 1 Airway epithelial cell Epithelial-to-mesenchymal transition Cell migration Collagen synthesis |
|
| 点击此处可从《Biocell》浏览原始摘要信息 |
|
点击此处可从《Biocell》下载全文 |
|