Effect of <i>Lycopus lucidus</i> Turcz. supplementation on gut microflora and short chain fatty acid composition in Crj: CD-1 mice |
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Authors: | EUN NA SUN YOUNG LIM |
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Affiliation: | 1.Ocean Science and Technology School, Korea Maritime & Ocean University, Busan, Korea
2 Division of Convergence on Marine Science, Korea Maritime & Ocean University, Busan, Korea |
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Abstract: | We investigated the diversity and composition of microflora in feces of Lycopus lucidus Turcz.-fed mice. In
addition, we evaluated the production of major cytokines (Interleukin-6 and -10) which are related to inflammation
and fatty acid composition of several tissues. 16S ribosomal DNA sequencing-based microbiome taxonomic profiling
analysis was performed utilizing the EzBioCloud data base. Male mice fed on L. lucidus showed a significantly
reduced number of lactic acid bacteria and coliform in the feces compared with the control group (p < 0.05). 16S
rDNA sequencing analysis of fecal samples showed that L. lucidus supplementation decreased the community of
harmful microflora (Enterobacteriaceae including Escherichia coli and Bacteroides sp.) in feces compared with the
control group (p < 0.05). There were no significant differences in mRNA expression of cytokine IL-6 and IL-10
between the control and L. lucidus fed groups. The fecal fatty acid composition in the L. lucidus group had
percentages of 4:0, 6:0, 8:0 and 10:0 in the intestine but those short chain fatty acids were not detected in the control
group. Our results showed that L. lucidus supplementation influenced gut environment by decreasing harmful
microflora and increased the percentages of several short fatty acids. |
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Keywords: | Lycopus lucidus Turcz Fatty acid composition Intestinal microflora 16S rDNA sequencing Lycopus lucidus Turcz" target="_blank">Cytokines" target="_blank">Lycopus lucidus Turcz Fatty acid composition Intestinal microflora 16S rDNA sequencing Cytokines |
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