通过式固相萃取结合UPLC-MS/MS同时测定烟用水基胶中的烷基酚、烷基酚聚氧乙烯醚和异噻唑啉酮

目的]建立通过式固相萃取结合超高效液相色谱?串联质谱(UPLC?MS/MS)同时测定烟用水基胶中烷基酚、烷基酚聚氧乙烯醚和异噻唑啉酮杀菌剂的方法.方法]样品用甲醇提取,经PRiME HLB通过式固相萃取净化后,以ACQUITY UPLC HSS C18 SB(100 mm×3.0 mm,1.8μm)为色谱柱,水和甲...

Simultaneous determination of alkylphenols,alkylphenol polyoxyethylene ethers and isothiazolinones in water-based adhesives of cigarette by ultra performance liquid chromatography-tandem mass spectrometry combined with passthrough SPE

  Objective  To realize the determination of alkylphenols, alkylphenol ethoxylatesand isothiazolinones in water-based adhesives of cigarette, ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analytical method combined with pass-through solid phase extraction was established.  Methods  The sample was extracted with methanol and purified by a PRiME HLB pass-through solid-phase extraction, followed by separation treatment with ACQUITY UPLC HSS C18 SB (100 mm×3.0 mm, 1.8 μm) chromatographic column using water and methanol as mobile phases.  Results  The results showed that: within 12 minutes, all analytes were eluted; under optimized conditions, the recoveries for all compounds were 80.8%-95.5%, with relative standard deviations (RSDs) less than 7.0%. Good coefficients of determination (R2≥0.9991) were achieved for all studied analytes. The LOD for all analytes varied from 0.17-0.62 mg/kg and the LOQ varied from 0.54-1.94 mg/kg.  Conclusion  The results confirm that the proposed method is stable and reliable, and can be used to determine alkylphenols, alkylphenol polyoxyethylene ethers and isothiazolinones in water-based adhesives of cigarette.