UPLC-MS/MS法检测电子烟烟液和气溶胶中的8种酚类化合物

【目的】建立一种定量检测电子烟烟液和气溶胶中8种潜在有害酚类化合物的方法，应用于电子烟产品的日常品质监管中。【方法】方法学的建立以电子烟烟液为样本，电子烟烟液经50%甲醇稀释后，利用液相-串联质谱联用仪(UPLC-MS/MS)测定，考察该方法的专属性、线性、检测限和定量限、准确度、精密度、以及溶液稳定性等。【结果】本方法中苯二酚、双酚A和剩余6种酚类化合物浓度分别在10～500μg/mL(R²=0.994)、0.02～1.00μg/mL(R²=0.998)和0.2～10.0μg/mL(R²=0.995～0.999)范围内时，线性关系良好；重复性测试溶液中各成分的RSD范围为3.6%～6.7%；各浓度加标溶液回收率为80.0%～119.2%；不同存储条件下样本稳定性良好。【结论】本方法检测速度快、专属性高、稳定性强，可用于准确有效检测电子烟烟液和气溶胶中的8种酚类化合物，并能高效应用于电子烟产品的品质管控中。

Detection of 8 phenolic compounds in E-cigarette liquids and aerosols by UPLC-MS/MS method

  Objective  This study aims to establish a qualitative and quantitative method for the determination of 8 potentially harmful phenolic compounds in e-cigarette liquids and aerosols, and apply it to the daily quality control of e-cigarettes.  Methods  The e-cigarette liquid samples were firstly diluted with 50% methanol and then determined by liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The specificity, linearity, limit of detection and quantification, accuracy, precision, recovery rate and stability of the method were investigated.  Objective  The suitable linear ranges of hydroquinone, bisphenol A and the remaining six phenolic compounds were 10-500 μg/mL (R2 = 0.994), 0.02-1.00 μg/mL (R2 = 0.998) and 0.2-10.0 μg/mL (R2 = 0.995-0.999), respectively. The relative recovery rates ranged from 80.0% to 119.2%. The maximum RSD of each component in repeatable test solution was 3.6%-6.7%; The stability of samples under different storage conditions was good.  Methods  This method is rapid, specific, accurate and stable in detection of 8 phenolic compounds in e-cigarette liquids and aerosols.