酶抑制分光光度法与表面增强拉曼散射在农药检测中的进展

实现有机磷农药残留的快速检测,对农产品质量安全和人民生命健康具有重要意义。本文针对有机磷农药快速检测中的酶抑制分光光度法和表面增强拉曼光谱技术(SERS)分别就研究进展、应用情况以及面临的问题进行综述,旨在为后续有机磷农药快速检测的研究人员提供技术与应用参考。酶抑制分光光度法可对样品中有机磷农药残留总量做粗略估计,主要应用于大量样品进行快速筛选,但在检测过程中,存在酶活性一致性差、易受检测对象干扰以及检测环境严格等缺点。SERS技术具有指纹特性、检测速度快、检测成本低以及检测精度高等优点,可对样品中的有机磷农药残留进行快速定性定量分析,具有巨大的应用潜力。当然,作为新兴分析手段,在重复与稳定性上仍面临一些问题。

Progress of Enzyme Inhibition Spectrophotometry and Surface Enhanced Raman Scatter for Detection of Pesticides

Rapid detection of organophosphorus pesticide is of great significance for the quality and safety of agricultural products and the people's health. The paper summarizes the research progress, application situation and problems of two detection methods of organophosphorus pesticide. They are the most widely detection method of organophosphorus pesticide-enzyme inhibition spectrophotometry and the great application prospect method-surface enhanced Raman scatter (SERS) technology. The work aims at providing the subsequent researcher the technology and application reference on the detection of organophosphorus pesticide. Firstly, the enzyme inhibition spectrophotometry is for the rough and total estimate of the organophosphorus pesticide residues and realize the rapid screening of a large number of the samples. But it has the shortcomings of the poor consistency of the enzyme activity and stringent testing environment, and it is also susceptible to some objects. Secondly, taking the advantages of fingerprint characteristics, high detection speed and precision, and low cost into consideration, the surface enhanced Raman scatter technology has great potential for the qualitative and quantitative analysis the pesticide residues in the samples. Of course, as a new analysis method, it is still faced with some problems of the repetition and stability.