Fatty acid analysis of blood serum, seminal plasma, and spermatozoa of normozoospermic vs. Asthernozoospermic males |
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Authors: | Julie A Conquer James B Martin Ian Tummon Lynn Watson Francis Tekpetey |
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Affiliation: | (1) Human Nutraceutical Research Unit and Depatment of Human Biology and Nutritional Science, University of Guelph, N1G 2W1 Guelph, Ontario, Canada;(2) London Health Sciences Centre and Department of Obstetrics and Gyencology, University of Western Ontario, N6A 5A5 London, Ontario, Canada |
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Abstract: | Docosahexaenoic acid (DHA; 22∶6n−3) is found in extremely high levels in human ejaculate with the majority occurring in the
spermatozoa. However, the relative concentration of DHA and other fatty acids, in blood serum, seminal plasma, and spermatozoa
of asthenozoospermic vs. normozoospermic individuals is not known. We analyzed the phospholipid fatty acid composition of
blood serum, seminal plasma, and spermatozoa of normozoospermic men and asthenozoospermic men in order to determine if DHA
levels, as well as the levels of other fatty acids, differed. The serum phospholipid DHA levels were similar in the two groups,
suggesting similar intakes of dietary DHA. On the other hand, seminal plasma levels of DHA (3.0 vs. 3.7%) and total polyunsaturated
fatty acids (PUFA) (11.8 vs. 13.5%) were significantly lower in asthenozoospermic vs. normozoospermic men, respectively, while
18∶1 (19.0 vs. 16.8%) and monounsaturated fatty acids (MUFA) (24.2 vs. 21.7%) were significantly higher in the asthenozoospermic
vs. the normozoospermic men. Spermatozoa from asthenozoospermic men had higher levels of 18∶1, 20∶0, 22∶0, 22∶1, and 24∶0
than sperm from normozoospermic men, and lower levels of 18∶0 and DHA (8.2 vs. 13.8%). Furthermore, total MUFA (19.3 vs. 16.5%)
was higher and total PUFA (19.0 vs. 24.0%), n−3 fatty acids (9.3 vs. 14.6%), and the ratio of n−3 to n−6 fatty acids (1.0
vs. 1.6) were lower in the asthenozoospermic men. Therefore, in asthenozoospermic individuals, lower levels of DHA in the
seminal plasma, but not in the blood serum, mimic the decreased concentrations of DHA in the spermatozoa. This suggests that
the lower concentrations of spermatozoon DHA in these individuals are due not to dietary differences but to some type of metabolic
difference in the asthenozoopermic men. |
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