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Immersion oil for high‐resolution live‐cell imaging at 37°C: optical and physical characteristics |
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| Authors: | L.C.J.M. OOMEN R. SACHER H.H.J. BROCKS J.M. ZWIER G.J. BRAKENHOFF K. JALINK |
| Affiliation: | 1. Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands;2. Cargille Laboratories, 55 Commerce Rd., Cedar Grove, NJ 07009, U.S.A.;3. Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 316, 1098 SM Amsterdam, The Netherlands |
| Abstract: | The use of normal immersion oil, developed for 23°C, at 37°C greatly compromises both axial resolution and signal intensity. We developed and characterized an immersion oil for optimal performance in live‐cell imaging at 37°C. We quantify the improvements in resolution and intensity obtained when using the new oil instead of its standard 23°C counterparts. |
| Keywords: | Confocal microscopy fluorescence microscopy high‐resolution– high NA objectives live‐cell imaging refractive index mismatch |