Synthesis and in vitro digestion of resistant starch type III from enzymatically hydrolysed cassava starch |
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Authors: | Calvin Onyango Christopher Mutungi |
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Affiliation: | Kenya Industrial Research and Development Institute, PO Box 30650‐00100, Nairobi, Kenya |
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Abstract: | Resistant starch type III (RS III) was synthesised from cassava starch by autoclaving followed by debranching with pullulanase, at varied concentrations (0.4–12 U g?1) and times (2–8 h), and recrystallisation (?18 to 90 °C for 1–16 h). The highest RS III yield (22 g/100 g) was obtained at an enzyme concentration of 4 U g?1 after 8 h incubation, followed by recrystallisation at 25 °C for 16 h. Varying the recrystallisation conditions indicated that higher RS III yields (30–35 g/100 g) could be obtained at 90 °C within 2 h. Thinning cassava starch using α‐amylase prior to debranching using pullulanase did not further increase the RS III content. In vitro digestion data showed that whereas 44% RS III was digested after 6 h, the corresponding value for cassava starch was 89%. |
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Keywords: | α ‐Amylase cassava starch in vitro digestion pullulanase resistant starch type III |
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