| 反相液相色谱法测定杜仲叶中绿原酸 |
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| 引用本文： | 万春花,倪永年,龙洲雄,胡海山,鄢兵. 反相液相色谱法测定杜仲叶中绿原酸[J]. 食品科学, 2007, 28(1): 255-257 |
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| 作者姓名： | 万春花 倪永年 龙洲雄 胡海山 鄢兵 |
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| 作者单位： | 南昌大学化学系； 江西省分析测试研究所； 江西省分析测试研究所 江西南昌330047江西省分析测试研究所； 江西南昌330029； 江西南昌330047； |
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| 摘 要： | 通过正交试验选择杜仲叶中绿原酸的最佳提取条件:30%甲醇回流20min,用反相液相色谱法测定绿原酸含量:色谱柱为KromasilC18柱,流动相为甲醇-0.02%磷酸溶液(体积比22:78),波长327nm,流速1.0ml/min。绿原酸在9.68～242μg/ml呈良好线性关系,相关系数r为0.9999,本法快速、简单、准确、分离度好、回收率大于95.9%。
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| 关 键 词： | 杜仲叶 绿原酸 反相液相色谱法 正交试验 |
| 文章编号： | 1002-6630（2007）01-0255-03 |
| 修稿时间： | 2005-11-14 |
Determination of Chlorogenic Acid in Eucommia Ulmoides Olive Leaves by Reversed-Phase High Performance Liquid Chromatography |
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| WAN Chun-hua,NI Yong-nian,LONG Zhou-xiong,HU Hai-shan,YAN Bing. Determination of Chlorogenic Acid in Eucommia Ulmoides Olive Leaves by Reversed-Phase High Performance Liquid Chromatography[J]. Food Science, 2007, 28(1): 255-257 |
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| Authors: | WAN Chun-hua NI Yong-nian LONG Zhou-xiong HU Hai-shan YAN Bing |
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| Affiliation: | 1.Department of Chemistry, Nanchang University, Nanchang 330029, China; 2.Jiangxi Institute of Analysing and Testing , Nanchang 330029, China |
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| Abstract: | The conditions for the extracting chlorogenic acid in the Eucommia Ulmoides Olive leaves were optimized by the orthogonal test as follows: continuous extraction by 30% methanol for 20 minutes; determined by RP-HPLC. The chlorogenic acid was seperated on kromasil C18 column, with CH3OH～0.02%H3PO4 (22:78) at a flow-rate of 1.0ml/min and detected at UV 327nm.The calibration curve was linear in the range of 9.68～242μg/ml (r=0.9999). This method is simple, rapid and accurate with good separation, and the recoveries are above 95.9%. |
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| Keywords: | Eucommia Ulmoides Olive leaves chlorogenic acid reversed-phase high performance liquid chromatography (RP-HPLC) orthogonal test |
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