| 一株伯克霍尔德菌所产类细菌素的分离纯化 |
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| 引用本文： | 刘静,杨富民,王丽君,苏阿伦. 一株伯克霍尔德菌所产类细菌素的分离纯化[J]. 中国酿造, 2021, 40(1): 138-141. DOI: 10.11882/j.issn.0254-5071.2021.01.026 |
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| 作者姓名： | 刘静 杨富民 王丽君 苏阿伦 |
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| 作者单位： | （1.甘肃农业大学 食品科学与工程学院，甘肃 兰州 730070；2.甘肃省食品检验研究院，甘肃 兰州 730030） |
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| 基金项目： | 甘肃省科技计划资助(20JR5RA168)。 |
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| 摘 要： | 利用硫酸铵分级沉淀、固相萃取小柱层析、高效液相色谱分离的方法纯化一株伯克霍尔德菌所产类细菌素。结果表明，纯化效果较好，在保留时间5.783 min时出现一个较强的单一吸收峰，抑菌物质的最大吸收波长为223.06 nm，蛋白质含量为452 μg/mL。
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| 关 键 词： | 固相萃取小柱 高效液相色谱法 纯化 伯克霍尔德菌 |
Isolation and purification of bacteriocin-like produced by Burkholderia |
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| LIU Jing,YANG Fumin,WANG Lijun,SU Alun. Isolation and purification of bacteriocin-like produced by Burkholderia[J]. China Brewing, 2021, 40(1): 138-141. DOI: 10.11882/j.issn.0254-5071.2021.01.026 |
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| Authors: | LIU Jing YANG Fumin WANG Lijun SU Alun |
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| Affiliation: | (1.College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China; 2.Gansu Province Food Inspection Institute, Lanzhou 730030, China) |
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| Abstract: | The bacteriocin-like produced by Burkholderia were purified by ammonium sulfate precipitation,solid phase extraction and high performance liquid chromatography(HPLC)isolation.The results showed that the purification effect was good,and a strong single absorption peak appeared at the retention time of 5.783 min.The maximum absorption wavelength of antibacterial substances was 223.06 nm,and the protein content was 452μg/ml. |
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| Keywords: | SPE column reverse HPLC purification Burkholderia |
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