清香型白酒酒醅发酵菌株分离鉴定及细菌群落结构分析

采用纯培养技术对清香型白酒酒醅中的乳酸菌和芽孢杆菌进行分离鉴定，同时通过Illumina MiSeq高通量测序技术解析清香型白酒酒醅细菌群落结构。结果表明，所有酒醅样品共分离得到13株乳酸菌，分别鉴定为乳酸片球菌（Pediococcus acidilactici）4株、副干酪乳杆菌（Lactobacillus paracasei）3株、短乳杆菌（Lactobacillus brevis）3株、粪肠球菌（Enterococcus faecium）2株以及希尔氏乳杆菌（Lactobacillus hilgardii）1株。从酒醅中分离得到的17株芽孢杆菌菌株分别鉴定为地衣芽孢杆菌（Bacillus licheniformis）9株、枯草芽孢杆菌（Bacillus subtilis）2株、短小芽胞杆菌（Bacillus pumilus）2株、澳洲芽孢杆菌（Bacillus australimaris）2株、解淀粉芽孢杆菌（Bacillus amyloliquefaciens）1株以及蜡样芽孢杆菌（Bacillus cereus）1株。Illumina MiSeq高通量测序技术结果显示，酒醅中优势菌门分别为厚壁菌门（Firmicutes）（97.96%）、放线菌门（Actinobacteria）（1.24%）和变形菌门（Proteobacteria）（0.76%）；优势细菌属为乳酸杆菌属（Lactobacillus）（97.42%）、栖热嗜油菌属（Thermoleophilum）（1.22%）和假单胞菌属（Pseudomonas）（0.72%）。

Isolation and identification of fermentation strains and analysis of bacterial community structure in fermented grains of light-flavor Baijiu

Lactic acid bacteria and Bacillus were isolated and identified in fermented grains of light-flavor Baijiu(Chinese liquor)by pure culture technology,and the bacterial community structure was analyzed by Illumina MiSeq high-throughput sequencing technology.The results showed that a total of 13 strains of lactic acid bacteria were isolated from all samples,which were identified as Pediococcus acidilactici(4 strains),Lactobacillus paracasei(3 strains),Lactobacillus brevis(3 strains),Enterococcus faecium(2 strains)and Lactobacillus hilgardii(1 strain).Seventeen strains of Bacillus isolated from fermented grains of light-flavor Baijiu were identified as Bacillus licheniformis(9 strains),Bacillus subtilis(2 strains),Bacillus pumilus(2 strains),Bacillus australimaris(2 strains),Bacillus amyloliquefaciens(1 strain),and Bacillus cereus(1 strain).Illumina MiSeq high-throughput sequencing technology revealed that the dominant bacteria in the fermented grains were Firmicutes(97.96%),Actinobacteria(1.24%)and Proteobacteria(0.76%),and the dominant genera were Lactobacillus(97.42%),Thermoleophilum(1.22%)and Pseudomonas(0.72%).